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Literature summary extracted from

  • Yi, S.Y.; Cui, Y.; Zhao, Y.; Liu, Z.D.; Lin, Y.J.; Zhou, F.
    A novel naturally occurring class I 5-enolpyruvylshikimate-3-phosphate synthase from Janibacter sp. confers high glyphosate tolerance to rice (2016), Sci. Rep., 6, 19104.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expressed in Escherichia coli AB2829 cells Janibacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.19 agriculture the enzyme is efficiently utilized to develop transgenic glyphosate-tolerant crops Janibacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 glyphosate
-
 Janibacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.03
-
 phosphoenolpyruvate at pH 8.0 and 40°C Janibacter sp.
2.5.1.19 0.083
-
 3-phosphoshikimate at pH 8.0 and 40°C Janibacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 47000
-
 x * 47000, SDS-PAGE Janibacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Janibacter sp.
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Janibacter sp. A3TGI7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Janibacter sp. phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.19 ? x * 47000, SDS-PAGE Janibacter sp.

Synonyms

EC Number Synonyms Comment Organism
2.5.1.19 5-enol-pyruvylshikimate 3-phosphate synthase
-
 Janibacter sp.
2.5.1.19 AroA
-
 Janibacter sp.
2.5.1.19 EPSPS
-
 Janibacter sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.373
-
 glyphosate at pH 8.0 and 40°C Janibacter sp.

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.19 0.04
-
 at pH 8.0 and 40°C Janibacter sp. glyphosate