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Literature summary extracted from

  • Teng, Y.B.; Jing, H.; Aramsangtienchai, P.; He, B.; Khan, S.; Hu, J.; Lin, H.; Hao, Q.
    Efficient demyristoylase activity of SIRT2 revealed by kinetic and structural studies (2015), Sci. Rep., 5, 8529.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.286 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.286 in complex with BHJH-TM1 protein, hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M HEPES buffer, pH 7.5 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.286 0.00024
-
 [histone H3]-N6-myristoyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens
2.3.1.286 0.019
-
 [histone H3]-N6-acetyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.286 NAD+ + [protein]-N6-acetyl-L-lysine Homo sapiens
-
 nicotinamide + [protein]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.286 NAD+ + [protein]-N6-myristoyl-L-lysine Homo sapiens demyristoylase activity of SIRT2 is 5fold more efficient than its deacetylase activity nicotinamide + [protein]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.286 Homo sapiens Q8IXJ6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.286 Ni-NTA column chromatography and Superdex G200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.286 NAD+ + [BHJH-TM1 protein]-N6-myristoyl-L-lysine
-
 Homo sapiens nicotinamide + [BHJH-TM1 protein]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
2.3.1.286 NAD+ + [histone H2B]-N6-acetyl-L-lysine12
-
 Homo sapiens nicotinamide + [histone H2B]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.286 NAD+ + [histone H2B]-N6-myristoyl-L-lysine12
-
 Homo sapiens nicotinamide + [histone H2B]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
2.3.1.286 NAD+ + [histone H3]-N6-acetyl-L-lysine9
-
 Homo sapiens nicotinamide + [histone H3]-L-lysine9 + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.286 NAD+ + [histone H3]-N6-myristoyl-L-lysine9
-
 Homo sapiens nicotinamide + [histone H3]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
2.3.1.286 NAD+ + [histone H4]-N6-acetyl-L-lysine16
-
 Homo sapiens nicotinamide + [histone H4]-L-lysine16 + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.286 NAD+ + [histone H4]-N6-myristoyl-L-lysine16
-
 Homo sapiens nicotinamide + [histone H4]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
2.3.1.286 NAD+ + [protein]-N6-acetyl-L-lysine
-
 Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.286 NAD+ + [protein]-N6-myristoyl-L-lysine demyristoylase activity of SIRT2 is 5fold more efficient than its deacetylase activity Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.286 SIRT2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.286 0.018
-
 [histone H3]-N6-myristoyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens
2.3.1.286 0.275
-
 [histone H3]-N6-acetyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.286 NAD+ dependent on Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.286 14.5
-
 [histone H3]-N6-acetyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens
2.3.1.286 74
-
 [histone H3]-N6-myristoyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens