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Literature summary extracted from

  • Li, B.; Tempel, W.; Smil, D.; Bolshan, Y.; Schapira, M.; Park, H.W.
    Crystal structures of Klebsiella pneumoniae pantothenate kinase in complex with N-substituted pantothenamides (2013), Proteins, 81, 1466-1472.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 expressed in Escherichia coli BL21(DE3) cells Klebsiella pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.33 in complex with N-pyridin-3-ylmethylpantothenamide, sitting drop vapor diffusion method, using 20% (w/v) PEG3350 and 0.2 M tri-lithium citrate Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate Klebsiella pneumoniae
-
 ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate Klebsiella pneumoniae 342
-
 ADP + (R)-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Klebsiella pneumoniae B5XYG3
-
-
2.7.1.33 Klebsiella pneumoniae 342 B5XYG3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33 Ni-NTA resin column chromatography and Superdex 75 gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Klebsiella pneumoniae ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Klebsiella pneumoniae 342 ADP + (R)-4'-phosphopantothenate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 PanK
-
 Klebsiella pneumoniae