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Literature summary extracted from

  • Danley, D.E.; Baima, E.T.; Mansour, M.; Fennell, K.F.; Chrunyk, B.A.; Mueller, J.P.; Liu, S.; Qiu, X.
    Discovery and structural characterization of an allosteric inhibitor of bacterial cis-prenyltransferase (2015), Protein Sci., 24, 20-26.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.31 gene uppS, recombinant His-tagged enzyme expression in Escherichia coli strain BL21 Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.31 purified recombinant enzyme free or in complex with substrate (2E,6E)-farnesyl diphosphate, X-ray diffraction structure determination and analysis at resolutions of 2.2 A and 2.1A, respectively Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.31 3-(2-chlorophenyl)-N-(4-isopropylphenyl-5-methylisoxazole-4-carboxamide) i.e. UK-106051, a drug-like UPPs inhibitor, UK-106051 binds to a site in the middle of this binding cleft. Substrates FPP and IPP cannot bind simultaneously to UPPs-inhibitor binary complexes Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.31 Mg2+ required Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.31 (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Streptococcus pneumoniae
-
 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.31 Streptococcus pneumoniae Q8DRB3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.31 recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.31 (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Streptococcus pneumoniae 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.31 undecaprenyl pyrophosphate synthase
-
 Streptococcus pneumoniae
2.5.1.31 UPPs
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.31 37
-
 assay at Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.31 7.5
-
 assay at Streptococcus pneumoniae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.31 0.00005
-
 pH 7.5, 37°C Streptococcus pneumoniae 3-(2-chlorophenyl)-N-(4-isopropylphenyl-5-methylisoxazole-4-carboxamide)

General Information

EC Number General Information Comment Organism
2.5.1.31 physiological function the enzyme is an essential enzyme in a key bacterial cell wall synthesis pathway. It catalyzes the consecutive condensations of isopentenyl diphosphate groups on to a trans-farnesyl diphosphate to produce the C55 isoprenoid undecaprenyl diphosphate Streptococcus pneumoniae