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Literature summary extracted from

  • Tang, M.A.; Motoshima, H.; Watanabe, K.
    Cold adaptation: structural and functional characterizations of psychrophilic and mesophilic acetate kinase (2014), Protein J., 33, 313-322.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.1 gene ackA, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499 Shewanella sp.
2.7.2.1 gene ackA, recombinant His-tagged enzyme overexpression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 additional information
-
 additional information Michaelis-Menten kinetics, thermodynamics Escherichia coli
2.7.2.1 additional information
-
 additional information Michaelis-Menten kinetics, thermodynamics Shewanella sp.
2.7.2.1 0.47
-
 ADP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.48
-
 ATP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.48
-
 ATP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.49
-
 ATP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.49
-
 ADP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.5
-
 acetyl phosphate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.5
-
 ATP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.5
-
 ADP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.5
-
 ADP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.53
-
 acetyl phosphate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.53
-
 acetyl phosphate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.53
-
 acetyl phosphate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 0.57
-
 ATP pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 0.59
-
 ATP pH 7.5, 25°C, recombinant enzyme Escherichia coli
2.7.2.1 0.59
-
 acetyl phosphate pH 7.5, 25°C, recombinant enzyme Escherichia coli
2.7.2.1 0.61
-
 ADP pH 7.5, 25°C, recombinant enzyme Escherichia coli
2.7.2.1 0.62
-
 ADP pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 0.64
-
 ATP pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 0.64
-
 ADP pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 0.65
-
 ATP pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 0.66
-
 ADP pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 0.67
-
 acetyl phosphate pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 0.69
-
 acetyl phosphate pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 0.71
-
 acetyl phosphate pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 1.61
-
 acetate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 1.63
-
 acetate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 1.71
-
 acetate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 1.77
-
 acetate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 29.3
-
 acetate pH 7.5, 25°C, recombinant enzyme Escherichia coli
2.7.2.1 31.32
-
 acetate pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 32.26
-
 acetate pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 36.02
-
 acetate pH 7.5, 10°C, recombinant enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Mg2+ required Shewanella sp.
2.7.2.1 Mg2+ the enzyme requires Mn2+ or Mg2+ Escherichia coli
2.7.2.1 Mn2+ the enzyme requires Mn2+ or Mg2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 42000
-
-
 Escherichia coli
2.7.2.1 43000
-
-
 Shewanella sp.
2.7.2.1 43300
-
-
 Escherichia coli
2.7.2.1 43700
-
-
 Shewanella sp.
2.7.2.1 86000
-
 gel filtration, recombinant enzyme Escherichia coli
2.7.2.1 86000
-
 gel filtration, recombinant enzyme Shewanella sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Escherichia coli
-
 ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate Shewanella sp.
-
 ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate Shewanella sp. AS-11
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Escherichia coli P0A6A3 gene ackA
-
2.7.2.1 Shewanella sp. E3W769 gene ackA
-
2.7.2.1 Shewanella sp. AS-11 E3W769 gene ackA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1 recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography Escherichia coli
2.7.2.1 recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography Shewanella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 acetyl phosphate + hydroxylamine
-
 Escherichia coli acetyl hydroxamate + phosphate
-
 ?
2.7.2.1 acetyl phosphate + hydroxylamine
-
 Shewanella sp. acetyl hydroxamate + phosphate
-
 ?
2.7.2.1 acetyl phosphate + hydroxylamine
-
 Shewanella sp. AS-11 acetyl hydroxamate + phosphate
-
 ?
2.7.2.1 ATP + acetate
-
 Escherichia coli ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate
-
 Shewanella sp. ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate the equilibrium lies far to the left side Escherichia coli ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate
-
 Shewanella sp. AS-11 ADP + acetyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.1 homodimer 2 * 42000, recombinant enzyme, SDS-PAGE, 2 * 43300, about, sequence calculation Escherichia coli
2.7.2.1 homodimer 2 * 43000, recombinant enzyme, SDS-PAGE, 2 * 43700, about, sequence calculation Shewanella sp.

Synonyms

EC Number Synonyms Comment Organism
2.7.2.1 ackA
-
 Escherichia coli
2.7.2.1 EAK
-
 Escherichia coli
2.7.2.1 Sak
-
 Shewanella sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.1 50
-
 about Shewanella sp.
2.7.2.1 70
-
 about Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.1 20 80 purified recombinant His-tagged enzyme, pH 7.5, 50% of maximal activity remaining after 15 min at 55°C for the free enzyme, at 65°C for the acetate-bound enzyme, and at 70°C for the ATP-bound enzyme. Free enzyme is completely stable up to 40°C, inactivation of all enzymes at 80°C Escherichia coli
2.7.2.1 20 80 purified recombinant His-tagged enzyme, pH 7.5, the enzyme is completely stable up to 30°C, half-life is about 40-50°C, inactivation at 80°C Shewanella sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.1 195
-
 acetate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 205
-
 ATP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 235
-
 ATP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 245
-
 acetate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 300
-
 acetate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 305
-
 ATP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 350
-
 ATP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 385
-
 acetate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 560
-
 acetate pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 565
-
 ATP pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 745
-
 ATP pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 760
-
 acetate pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 875
-
 acetyl phosphate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 884
-
 ADP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 890
-
 acetyl phosphate pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 892
-
 ADP pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 1130
-
 ATP pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 1165
-
 acetate pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 1340
-
 acetyl phosphate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 1350
-
 ADP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 1420
-
 acetate pH 7.5, 25°C, recombinant enzyme Escherichia coli
2.7.2.1 1500
-
 ATP pH 7.5, 25°C, recombinant enzyme Escherichia coli
2.7.2.1 1550
-
 acetyl phosphate pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 1565
-
 ADP pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 1700
-
 ADP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 1700
-
 acetyl phosphate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 2100
-
 acetyl phosphate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 2135
-
 ADP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 2150
-
 acetyl phosphate pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 2178
-
 ADP pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 3515
-
 ADP pH 7.5, 25°C, recombinant enzyme Escherichia coli
2.7.2.1 3600
-
 acetyl phosphate pH 7.5, 25°C, recombinant enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 7.5
-
 assay at Escherichia coli
2.7.2.1 7.5
-
 assay at Shewanella sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.1 ADP
-
 Escherichia coli
2.7.2.1 ADP
-
 Shewanella sp.
2.7.2.1 ATP
-
 Escherichia coli
2.7.2.1 ATP
-
 Shewanella sp.

General Information

EC Number General Information Comment Organism
2.7.2.1 evolution the enzyme is found in anaerobic as well as aerobic bacteria and Archaea. The enzyme belongs to the ASKHA phosphotransferase family (acetate and sugar kinases/Hsc70/actin) and is the best candidate for the common ancestor of this family Shewanella sp.
2.7.2.1 evolution the enzyme is found in anaerobic as well as aerobic bacteria and domain of Archaea, and play a central role in major link in the carbon cycle. The enzyme is also participated in the production of major amount o ATP in most of the fermentative anaerobes. The enzyme belongs to the phosphotransferases of the ASKHA (acetate and sugar kinases/Hsc70/actin) and the best candidate for the common ancestor of this family Escherichia coli
2.7.2.1 metabolism the enzyme plays a central role in major link in the carbon cycle. The enzyme also participates in the production of major amount of ATP in most of the fermentative anaerobes Shewanella sp.
2.7.2.1 additional information salts bridges and cation-pi interactions in the enzyme, overview Escherichia coli
2.7.2.1 additional information salts bridges and cation-pi interactions in the enzyme, overview Shewanella sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.2.1 15.5
-
 acetate pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 23.5
-
 acetate pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 37.2
-
 acetate pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 48.5
-
 acetate pH 7.5, 25°C, recombinant enzyme Escherichia coli
2.7.2.1 127
-
 acetate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 144
-
 acetate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 178
-
 acetate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 198
-
 acetate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 390
-
 ATP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 510
-
 ATP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 625
-
 ATP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 785
-
 ATP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 869
-
 ATP pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 1164
-
 ATP pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 1290
-
 acetyl phosphate pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 1351
-
 ADP pH 7.5, 10°C, recombinant enzyme Escherichia coli
2.7.2.1 1650
-
 acetyl phosphate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 1768
-
 ADP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
2.7.2.1 1982
-
 ATP pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 2183
-
 acetyl phosphate pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 2445
-
 ADP pH 7.5, 15°C, recombinant enzyme Escherichia coli
2.7.2.1 2528
-
 acetyl phosphate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 2542
-
 ATP pH 7.5, 25°C, recombinant enzyme Escherichia coli
2.7.2.1 2700
-
 ADP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2.7.2.1 3207
-
 acetyl phosphate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 3209
-
 acetyl phosphate pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 3470
-
 ADP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2.7.2.1 3513
-
 ADP pH 7.5, 20°C, recombinant enzyme Escherichia coli
2.7.2.1 4200
-
 acetyl phosphate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 4542
-
 ADP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2.7.2.1 5762
-
 ADP pH 7.5, 25°C, recombinant enzyme Escherichia coli
2.7.2.1 6102
-
 acetyl phosphate pH 7.5, 25°C, recombinant enzyme Escherichia coli