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Literature summary extracted from

  • Cheng, F.; Hu, T.; An, Y.; Huang, J.; Xu, Y.
    Purification and enzymatic characterization of alcohol dehydrogenase from Arabidopsis thaliana (2013), Protein Expr. Purif., 90, 74-77.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 1.65
-
NAD+ pH 10.5, 25°C Arabidopsis thaliana
1.1.1.1 5.1
-
ethanol pH 10.5, 25°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 40700
-
-
Arabidopsis thaliana
1.1.1.1 70000 80000 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 butan-1-ol + NAD+ 90.7% of the activity with ethanol Arabidopsis thaliana butanal + NADH + H+
-
?
1.1.1.1 ethanol + NAD+
-
Arabidopsis thaliana acetaldehyde + NADH + H+
-
?
1.1.1.1 isopentan-1-ol + NAD+ 34% of the activity with ethanol Arabidopsis thaliana isopentanal + NADH + H+
-
?
1.1.1.1 pentan-1-ol + NAD+ 75.6% of the activity with ethanol Arabidopsis thaliana pentanal + NADH + H+
-
?
1.1.1.1 propan-1-ol + NAD+ 89.3% of the activity with ethanol Arabidopsis thaliana propanal + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 40700, calculated Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 10.5
-
-
Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 8.5
-
more than 50% of maximum activity Arabidopsis thaliana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.1 11
-
rapid loss of activity Arabidopsis thaliana