EC Number | Cloned (Comment) | Organism |
---|---|---|
2.9.1.1 | expressed in Escherichia coli strain WL81460 | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.9.1.1 | 50000 | - |
- |
Escherichia coli |
2.9.1.1 | 600000 | - |
gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.9.1.1 | L-seryl-tRNASec + selenophosphate | Escherichia coli | - |
L-selenocysteinyl-tRNASec + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.9.1.1 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.9.1.1 | ammonium sulfate precipitation, hydroxyapatite column chromatography, Hi Trap Q column chromatography, and Superdex 200 gel filtration | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.9.1.1 | L-seryl-tRNASec + selenophosphate | - |
Escherichia coli | L-selenocysteinyl-tRNASec + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.9.1.1 | homodecamer | 10 * 50000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.9.1.1 | SelA | - |
Escherichia coli |
2.9.1.1 | Selenocysteine synthase | - |
Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.9.1.1 | 45 | - |
the protein stability is maintained up to 45°C. The enzyme has a thermal transition from 60 to 70°C with a melting temperature of 66°C and an evident plateau above 70°C, in which all secondary structures are lost and the protein is completely denatured | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.9.1.1 | pyridoxal 5'-phosphate | - |
Escherichia coli |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.9.1.1 | Escherichia coli | isoelectric focusing | - |
6 |