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Literature summary extracted from

  • Zhang, S.; Wang, L.; Liu, Y.; Zhai, H.; Cai, J.; Hu, Y.
    Expression of feruloyl esterase A from Aspergillus terreus and its application in biomass degradation (2015), Protein Expr. Purif., 115, 153-157.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 degradation the hydrolysis of corn stalk and corncob by xylanase from Aspergillus niger can be significantly improved in concert with recombinant FaeA Aspergillus terreus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Pichia pastoris Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.29
-
 sinapic acid methyl ester pH 5.0, 40°C Aspergillus terreus
3.1.1.73 0.38
-
 p-coumaric acid methyl ester pH 5.0, 40°C Aspergillus terreus
3.1.1.73 0.61
-
 ferulic acid methyl ester pH 5.0, 40°C Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 27000
-
-
 Aspergillus terreus
3.1.1.73 37000
-
-
 Aspergillus terreus
3.1.1.73 40000
-
-
 Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus terreus A0A068FNL1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.73 glycoprotein
-
 Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ferulic acid methyl ester + H2O
-
 Aspergillus terreus ferulic acid + methanol
-
 ?
3.1.1.73 p-coumaric acid methyl ester + H2O
-
 Aspergillus terreus p-coumaric acid + methanol
-
 ?
3.1.1.73 sinapic acid methyl ester + H2O
-
 Aspergillus terreus sinapic acid + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 40000 and x * 37000, differently glycosylated forms, x * 27000, deglycosylated form Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50
-
-
 Aspergillus terreus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.31
-
 p-coumaric acid methyl ester pH 5.0, 40°C Aspergillus terreus
3.1.1.73 0.94
-
 sinapic acid methyl ester pH 5.0, 40°C Aspergillus terreus
3.1.1.73 2.6
-
 ferulic acid methyl ester pH 5.0, 40°C Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
-
 Aspergillus terreus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 4.5 5.5 more than 90% of maximum activity Aspergillus terreus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 0.81
-
 p-coumaric acid methyl ester pH 5.0, 40°C Aspergillus terreus
3.1.1.73 3.23
-
 sinapic acid methyl ester pH 5.0, 40°C Aspergillus terreus
3.1.1.73 4.25
-
 ferulic acid methyl ester pH 5.0, 40°C Aspergillus terreus