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Literature summary extracted from

  • Choengpanya, K.; Arthornthurasuk, S.; Wattana-amorn, P.; Huang, W.T.; Plengmuankhae, W.; Li, Y.K.; Kongsaeree, P.T.
    Cloning, expression and characterization of beta-xylosidase from Aspergillus niger ASKU28 (2015), Protein Expr. Purif., 115, 132-140.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.37 synthesis upon codon optimization for expression in Pichia pastoris, the expression level increases to 5.7 g/l in a fermenter system Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expression in Pichia pastoris Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.39
-
 3,4-dinitrophenyl beta-D-xylopyranoside pH 4.0, 40°C Aspergillus niger
3.2.1.37 0.66
-
 4-nitrophenyl beta-D-xylopyranoside pH 4.0, 40°C Aspergillus niger
3.2.1.37 4.01
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.0, 40°C Aspergillus niger
3.2.1.37 4.54
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 4.0, 40°C Aspergillus niger
3.2.1.37 30.8
-
 xylobiose pH 4.0, 40°C Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Aspergillus niger A0A023J5W7
-
-
3.2.1.37 Aspergillus niger ASKU28 A0A023J5W7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 recombinant protein Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 3,4-dinitrophenyl beta-D-xylopyranoside + H2O
-
 Aspergillus niger 3,4-dinitrophenol + D-xylopyranose
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger ASKU28 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus niger 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus niger ASKU28 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Aspergillus niger 4-nitrophenol + D-xylopyranose
-
 ?
3.2.1.37 beechwood xylan + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.37 beechwood xylan + H2O
-
 Aspergillus niger ASKU28 ?
-
 ?
3.2.1.37 additional information the enzyme displays an exo-type activity with retention of the beta-configuration, and a synergistic action with xylanase in hydrolysis of beechwood xylan Aspergillus niger ?
-
 ?
3.2.1.37 additional information the enzyme displays an exo-type activity with retention of the beta-configuration, and a synergistic action with xylanase in hydrolysis of beechwood xylan Aspergillus niger ASKU28 ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Aspergillus niger 2 D-xylose
-
 ?
3.2.1.37 xylobiose + H2O
-
 Aspergillus niger ASKU28 2 D-xylose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 70
-
-
 Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 13.5
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.0, 40°C Aspergillus niger
3.2.1.37 67.8
-
 xylobiose pH 4.0, 40°C Aspergillus niger
3.2.1.37 93.8
-
 3,4-dinitrophenyl beta-D-xylopyranoside pH 4.0, 40°C Aspergillus niger
3.2.1.37 113
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 4.0, 40°C Aspergillus niger
3.2.1.37 334
-
 4-nitrophenyl beta-D-xylopyranoside pH 4.0, 40°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 4 4.5
-
 Aspergillus niger

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.37 2.2
-
 xylobiose pH 4.0, 40°C Aspergillus niger
3.2.1.37 3.4
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.0, 40°C Aspergillus niger
3.2.1.37 24.9
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 4.0, 40°C Aspergillus niger
3.2.1.37 242
-
 3,4-dinitrophenyl beta-D-xylopyranoside pH 4.0, 40°C Aspergillus niger
3.2.1.37 504
-
 4-nitrophenyl beta-D-xylopyranoside pH 4.0, 40°C Aspergillus niger