BRENDA - Enzyme Database

Purification and functional characterization of diadenosine 5,5'-P(1),P(4)-tetraphosphate phosphorylases from Mycobacterium smegmatis and Mycobacterium avium

Honda, N.; Kim, H.; Rimbara, E.; Kato, A.; Shibayama, K.; Mori, S.; Protein Expr. Purif. 112, 37-42 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.7.53
-
Mycolicibacterium smegmatis
2.7.7.53
expression in Escherichia coli
Mycobacterium avium
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Ca2+
2 mM, 38% of the activity with Mn2+
Mycobacterium avium
2.7.7.53
Ca2+
2 mM, 43% of the activity with Mn2+
Mycolicibacterium smegmatis
2.7.7.53
Co2+
2 mM, 89% of the activity with Mn2+
Mycobacterium avium
2.7.7.53
Co2+
2 mM, 60% of the activity with Mn2+
Mycolicibacterium smegmatis
2.7.7.53
Mg2+
2 mM, 98% of the activity with Mn2+
Mycobacterium avium
2.7.7.53
Mg2+
2 mM, 97% of the activity with Mn2+
Mycolicibacterium smegmatis
2.7.7.53
Mn2+
2 mM, best divalent activator
Mycobacterium avium
2.7.7.53
Mn2+
2 mM, best divalent activator
Mycolicibacterium smegmatis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
22500
-
x * 22500, calculated
Mycolicibacterium smegmatis
2.7.7.53
24500
-
-
Mycobacterium avium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.53
Mycobacterium avium
A0A0H3A0T9
-
-
2.7.7.53
Mycobacterium avium 104
A0A0H3A0T9
-
-
2.7.7.53
Mycolicibacterium smegmatis
A0QWG3
-
-
2.7.7.53
Mycolicibacterium smegmatis ATCC 700084
A0QWG3
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
14% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
2 ADP
major product
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
26% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
2 ADP
major product
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
14% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium 104
2 ADP
major product
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
26% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis ATCC 700084
2 ADP
major product
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
739577
Mycolicibacterium smegmatis
ADP + ATP
no formation of AMP observed
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
739577
Mycobacterium avium
ADP + ATP
no formation of AMP observed
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
739577
Mycobacterium avium 104
ADP + ATP
no formation of AMP observed
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
739577
Mycolicibacterium smegmatis ATCC 700084
ADP + ATP
no formation of AMP observed
-
-
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
53% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
GDP + GTP
major products
-
-
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
56% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
GDP + GTP
major products
-
-
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
53% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium 104
GDP + GTP
major products
-
-
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
56% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis ATCC 700084
GDP + GTP
major products
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
44% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
?
-
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
47% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
?
-
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
47% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium 104
?
-
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
44% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis ATCC 700084
?
-
-
-
?
2.7.7.53
P1,P5-bis(5'-guanosyl) pentaphosphate + phosphate
49% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
p4G + GDP
major products
-
-
?
2.7.7.53
P1,P5-bis(5'-guanosyl) pentaphosphate + phosphate
55% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
p4G + GDP
major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)-P4-(5'-deoxythymidyl) tetraphosphate + phosphate
68% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
ATP + dTDP
or ADP + dTTP, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)-P5-(5'-deoxythymidyl) pentaphosphate + phosphate
30% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
p4A + dTDP
or ADP + p4dT, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)-P5-(5'-guanosyl) pentaphosphate + phosphate
49% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
p4A + GDP
major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-deoxythymidyl) pentaphosphate + phosphate
31% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
p4A + dTDP
or ADP + p4dT, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-deoxythymidyl) tetraphosphate + phosphate
85% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
ATP + dTDP
or ADP + dTTP, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-guanosyl) pentaphosphate + phosphate
30% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
p4A + GDP
major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
80% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
ATP + GDP
or ADP plus GTP, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
93% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
ATP + GDP
or ADP plus GTP, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
93% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium 104
ATP + GDP
or ADP plus GTP, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
80% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis ATCC 700084
ATP + GDP
or ADP plus GTP, major products
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.7.53
?
x * 24500, calculated
Mycobacterium avium
2.7.7.53
?
x * 22500, calculated
Mycolicibacterium smegmatis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.53
-
Mycolicibacterium smegmatis
2.7.7.53
expression in Escherichia coli
Mycobacterium avium
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Ca2+
2 mM, 38% of the activity with Mn2+
Mycobacterium avium
2.7.7.53
Ca2+
2 mM, 43% of the activity with Mn2+
Mycolicibacterium smegmatis
2.7.7.53
Co2+
2 mM, 89% of the activity with Mn2+
Mycobacterium avium
2.7.7.53
Co2+
2 mM, 60% of the activity with Mn2+
Mycolicibacterium smegmatis
2.7.7.53
Mg2+
2 mM, 98% of the activity with Mn2+
Mycobacterium avium
2.7.7.53
Mg2+
2 mM, 97% of the activity with Mn2+
Mycolicibacterium smegmatis
2.7.7.53
Mn2+
2 mM, best divalent activator
Mycobacterium avium
2.7.7.53
Mn2+
2 mM, best divalent activator
Mycolicibacterium smegmatis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
22500
-
x * 22500, calculated
Mycolicibacterium smegmatis
2.7.7.53
24500
-
-
Mycobacterium avium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
14% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
2 ADP
major product
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
26% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
2 ADP
major product
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
14% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium 104
2 ADP
major product
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
26% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis ATCC 700084
2 ADP
major product
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
739577
Mycolicibacterium smegmatis
ADP + ATP
no formation of AMP observed
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
739577
Mycobacterium avium
ADP + ATP
no formation of AMP observed
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
739577
Mycobacterium avium 104
ADP + ATP
no formation of AMP observed
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
739577
Mycolicibacterium smegmatis ATCC 700084
ADP + ATP
no formation of AMP observed
-
-
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
53% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
GDP + GTP
major products
-
-
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
56% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
GDP + GTP
major products
-
-
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
53% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium 104
GDP + GTP
major products
-
-
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
56% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis ATCC 700084
GDP + GTP
major products
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
44% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
?
-
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
47% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
?
-
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
47% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium 104
?
-
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
44% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis ATCC 700084
?
-
-
-
?
2.7.7.53
P1,P5-bis(5'-guanosyl) pentaphosphate + phosphate
49% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
p4G + GDP
major products
-
-
?
2.7.7.53
P1,P5-bis(5'-guanosyl) pentaphosphate + phosphate
55% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
p4G + GDP
major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)-P4-(5'-deoxythymidyl) tetraphosphate + phosphate
68% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
ATP + dTDP
or ADP + dTTP, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)-P5-(5'-deoxythymidyl) pentaphosphate + phosphate
30% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
p4A + dTDP
or ADP + p4dT, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)-P5-(5'-guanosyl) pentaphosphate + phosphate
49% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
p4A + GDP
major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-deoxythymidyl) pentaphosphate + phosphate
31% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
p4A + dTDP
or ADP + p4dT, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-deoxythymidyl) tetraphosphate + phosphate
85% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
ATP + dTDP
or ADP + dTTP, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-guanosyl) pentaphosphate + phosphate
30% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
p4A + GDP
major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
80% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis
ATP + GDP
or ADP plus GTP, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
93% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium
ATP + GDP
or ADP plus GTP, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
93% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycobacterium avium 104
ATP + GDP
or ADP plus GTP, major products
-
-
?
2.7.7.53
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate
80% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate
739577
Mycolicibacterium smegmatis ATCC 700084
ATP + GDP
or ADP plus GTP, major products
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.7.53
?
x * 24500, calculated
Mycobacterium avium
2.7.7.53
?
x * 22500, calculated
Mycolicibacterium smegmatis