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Literature summary extracted from

  • Honda, N.; Kim, H.; Rimbara, E.; Kato, A.; Shibayama, K.; Mori, S.
    Purification and functional characterization of diadenosine 5,5'-P(1),P(4)-tetraphosphate phosphorylases from Mycobacterium smegmatis and Mycobacterium avium (2015), Protein Expr. Purif., 112, 37-42.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.53
-
 Mycolicibacterium smegmatis
2.7.7.53 expression in Escherichia coli Mycobacterium avium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.53 Ca2+ 2 mM, 38% of the activity with Mn2+ Mycobacterium avium
2.7.7.53 Ca2+ 2 mM, 43% of the activity with Mn2+ Mycolicibacterium smegmatis
2.7.7.53 Co2+ 2 mM, 60% of the activity with Mn2+ Mycolicibacterium smegmatis
2.7.7.53 Co2+ 2 mM, 89% of the activity with Mn2+ Mycobacterium avium
2.7.7.53 Mg2+ 2 mM, 97% of the activity with Mn2+ Mycolicibacterium smegmatis
2.7.7.53 Mg2+ 2 mM, 98% of the activity with Mn2+ Mycobacterium avium
2.7.7.53 Mn2+ 2 mM, best divalent activator Mycolicibacterium smegmatis
2.7.7.53 Mn2+ 2 mM, best divalent activator Mycobacterium avium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.53 22500
-
 x * 22500, calculated Mycolicibacterium smegmatis
2.7.7.53 24500
-
-
 Mycobacterium avium

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.53 Mycobacterium avium A0A0H3A0T9
-
-
2.7.7.53 Mycobacterium avium 104 A0A0H3A0T9
-
-
2.7.7.53 Mycolicibacterium smegmatis A0QWG3
-
-
2.7.7.53 Mycolicibacterium smegmatis ATCC 700084 A0QWG3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.53 P1,P3-bis(5'-adenosyl) triphosphate + phosphate 14% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium 2 ADP major product ?
2.7.7.53 P1,P3-bis(5'-adenosyl) triphosphate + phosphate 26% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis 2 ADP major product ?
2.7.7.53 P1,P3-bis(5'-adenosyl) triphosphate + phosphate 14% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium 104 2 ADP major product ?
2.7.7.53 P1,P3-bis(5'-adenosyl) triphosphate + phosphate 26% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis ATCC 700084 2 ADP major product ?
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Mycolicibacterium smegmatis ADP + ATP no formation of AMP observed ?
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Mycobacterium avium ADP + ATP no formation of AMP observed ?
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Mycobacterium avium 104 ADP + ATP no formation of AMP observed ?
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Mycolicibacterium smegmatis ATCC 700084 ADP + ATP no formation of AMP observed ?
2.7.7.53 P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate 53% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium GDP + GTP major products ?
2.7.7.53 P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate 56% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis GDP + GTP major products ?
2.7.7.53 P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate 53% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium 104 GDP + GTP major products ?
2.7.7.53 P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate 56% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis ATCC 700084 GDP + GTP major products ?
2.7.7.53 P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate 44% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis ?
-
 ?
2.7.7.53 P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate 47% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium ?
-
 ?
2.7.7.53 P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate 47% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium 104 ?
-
 ?
2.7.7.53 P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate 44% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis ATCC 700084 ?
-
 ?
2.7.7.53 P1,P5-bis(5'-guanosyl) pentaphosphate + phosphate 49% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis p4G + GDP major products ?
2.7.7.53 P1,P5-bis(5'-guanosyl) pentaphosphate + phosphate 55% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium p4G + GDP major products ?
2.7.7.53 P1-(5'-adenosyl)-P4-(5'-deoxythymidyl) tetraphosphate + phosphate 68% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium ATP + dTDP or ADP + dTTP, major products ?
2.7.7.53 P1-(5'-adenosyl)-P5-(5'-deoxythymidyl) pentaphosphate + phosphate 30% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis p4A + dTDP or ADP + p4dT, major products ?
2.7.7.53 P1-(5'-adenosyl)-P5-(5'-guanosyl) pentaphosphate + phosphate 49% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium p4A + GDP major products ?
2.7.7.53 P1-(5'-adenosyl)P4-(5'-deoxythymidyl) pentaphosphate + phosphate 31% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium p4A + dTDP or ADP + p4dT, major products ?
2.7.7.53 P1-(5'-adenosyl)P4-(5'-deoxythymidyl) tetraphosphate + phosphate 85% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis ATP + dTDP or ADP + dTTP, major products ?
2.7.7.53 P1-(5'-adenosyl)P4-(5'-guanosyl) pentaphosphate + phosphate 30% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis p4A + GDP major products ?
2.7.7.53 P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate 80% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis ATP + GDP or ADP plus GTP, major products ?
2.7.7.53 P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate 93% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium ATP + GDP or ADP plus GTP, major products ?
2.7.7.53 P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate 93% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium avium 104 ATP + GDP or ADP plus GTP, major products ?
2.7.7.53 P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate 80% of the activity with P1,P4-bis(5'-adenosyl) tetraphosphate Mycolicibacterium smegmatis ATCC 700084 ATP + GDP or ADP plus GTP, major products ?

Subunits

EC Number Subunits Comment Organism
2.7.7.53 ? x * 22500, calculated Mycolicibacterium smegmatis
2.7.7.53 ? x * 24500, calculated Mycobacterium avium

Synonyms

EC Number Synonyms Comment Organism
2.7.7.53 MAV_3489
-
 Mycobacterium avium
2.7.7.53 MEG_2932
-
 Mycolicibacterium smegmatis