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Literature summary extracted from

  • Auslender, E.L.; Dorion, S.; Dumont, S.; Rivoal, J.
    Expression, purification and characterization of Solanum tuberosum recombinant cytosolic pyruvate kinase (2015), Protein Expr. Purif., 110, 7-13.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40 expressed in Escherichia coli BL21(DE3) cells Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 2-oxoglutarate potent inhibitor Solanum tuberosum
2.7.1.40 citrate potent inhibitor Solanum tuberosum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.40 cytosol
-
 Solanum tuberosum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 58000
-
 x * 58000, SDS-PAGE Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.40 ATP + pyruvate Solanum tuberosum
-
 ADP + phosphoenolpyruvate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 Ni-NTA resinchromatography Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 1.33
-
 crude extract, at pH 7.0 and 37°C Solanum tuberosum
2.7.1.40 34.76
-
 after 26.14fold purification, at pH 7.0 and 37°C Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ATP + pyruvate
-
 Solanum tuberosum ADP + phosphoenolpyruvate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.1.40 ? x * 58000, SDS-PAGE Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.40 PKC1
-
 Solanum tuberosum
2.7.1.40 pyruvate kinase 1
-
 Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 40
-
-
 Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 25 75 more than 60% activity between 25 and 75°C, the enzyme retains 80% of its activity after 2 min at 75°C Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 6.5
-
-
 Solanum tuberosum