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Literature summary extracted from

  • Li, J.; Pei, X.; Zhang, S.; Wu, Z.
    Improving the thermostability of feruloyl esterase by DNA shuffling and site-directed mutagenesis (2015), Process Biochem., 50, 1783-1787.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 additional information generation of a chimeric library from four homologous FAEs originated from fungi by a DNA shuffling strategy. Chimeras contain DNA fragments encoding the wild-type FaeA from Aspergillus terreus, FaeA from Penicillium chrysogenum and FaeA from Aspergillus flavus, as well as the M11 mutant of FaeA from Aspergillus niger. Chimeras with enhanced thermal stability contain segments from 3 or 4 of the parental enzymes. The half-lives of inactivation of the chimeras at 65°C are increased by up to 22 fold when compared to the most stable parental FAE. The release of ferulic acid from steam-exploded corn stalk using the best chimera is enhanced by 12.8fold when compared to the best parental FAE Aspergillus niger
3.1.1.73 additional information generation of a chimeric library from four homologous FAEs originated from fungi by a DNA shuffling strategy. Chimeras contain DNA fragments encoding the wild-type FaeA from Aspergillus terreus, FaeA from Penicillium chrysogenum and FaeA from Aspergillus flavus, as well as the M11 mutant of FaeA from Aspergillus niger. Chimeras with enhanced thermal stability contain segments from 3 or 4 of the parental enzymes. The half-lives of inactivation of the chimeras at 65°C are increased by up to 22 fold when compared to the most stable parental FAE. The release of ferulic acid from steam-exploded corn stalk using the best chimera is enhanced by 12.8fold when compared to the best parental FAE Aspergillus terreus
3.1.1.73 additional information generation of a chimeric library from four homologous FAEs originated from fungi by a DNA shuffling strategy. Chimeras contain DNA fragments encoding the wild-type FaeA from Aspergillus terreus, FaeA from Penicillium chrysogenum and FaeA from Aspergillus flavus, as well as the M11 mutant of FaeA from Aspergillus niger. Chimeras with enhanced thermal stability contain segments from 3 or 4 of the parental enzymes. The half-lives of inactivation of the chimeras at 65°C are increased by up to 22 fold when compared to the most stable parental FAE. The release of ferulic acid from steam-exploded corn stalk using the best chimera is enhanced by 12.8fold when compared to the best parental FAE Penicillium chrysogenum
3.1.1.73 additional information generation of a chimeric library from four homologous FAEs originated from fungi by a DNA shuffling strategy. Chimeras contain DNA fragments encoding the wild-type FaeA from Aspergillus terreus, FaeA from Penicillium chrysogenum and FaeA from Aspergillus flavus, as well as the M11 mutant of FaeA from Aspergillus niger. Chimeras with enhanced thermal stability contain segments from 3 or 4 of the parental enzymes. The half-lives of inactivation of the chimeras at 65°C are increased by up to 22 fold when compared to the most stable parental FAE. The release of ferulic acid from steam-exploded corn stalk using the best chimera is enhanced by 12.8fold when compared to the best parental FAE Aspergillus flavus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus flavus R9UUB4
-
-
3.1.1.73 Aspergillus niger O42807
-
-
3.1.1.73 Aspergillus terreus Q0CBM7
-
-
3.1.1.73 Aspergillus terreus NIH 2624 Q0CBM7
-
-
3.1.1.73 Penicillium chrysogenum S5Q8E1
-
-

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAEA
-
 Aspergillus niger
3.1.1.73 FAEA
-
 Aspergillus terreus
3.1.1.73 FAEA
-
 Aspergillus flavus
3.1.1.73 XM 001217492
-
 Aspergillus terreus