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Literature summary extracted from

  • McLaughlin, M.I.; Lanz, N.D.; Goldman, P.J.; Lee, K.H.; Booker, S.J.; Drennan, C.L.
    Crystallographic snapshots of sulfur insertion by lipoyl synthase (2016), Proc. Natl. Acad. Sci. USA, 113, 9446-9450.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.8 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.1.8 hanging drop vapor diffusion method, using 0.1 M HEPES, pH 7.5, 12% (w/v) PEG 10000, 7.5% (v/v) 2-methyl-2,4-pentanediol Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.8 Mycobacterium tuberculosis P9WK91
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.8 Ni-NTA column chromatography and Sephacryl S-200 gel filtration Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.8.1.8 LipA
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.8 4Fe-4S-center
-
 Mycobacterium tuberculosis
2.8.1.8 S-adenosyl-L-methionine
-
 Mycobacterium tuberculosis