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Literature summary extracted from

  • Brinkmann-Chen, S.; Flock, T.; Cahn, J.; Snow, C.; Brustad, E.; McIntosh, J.; Meinhold, P.; Zhang, L.; Arnold, F.
    General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH (2013), Proc. Natl. Acad. Sci. USA, 110, 10946-10951.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.86 G50D/S52D mutant engineered for reversal in cofactor specificity for NADH over NADPH Methanococcus aeolicus
1.1.1.86 R48P/S51L/S52D mutant engineered for reversal in cofactor specificity for NADH over NADPH Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 R48P/S51L/S52D/R84A mutant engineered for reversal in cofactor specificity for NADH over NADPH Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.383 A71S/R76D/S78AD/Q110V mutant engineered for reversal in cofactor specificity for NADH over NADPH Shewanella sp.
1.1.1.383 A71S/R76D/S78D/Q110V mutant engineered for reversal in cofactor specificity for NADH over NADPH Escherichia coli
1.1.1.383 A71S/R76D/S78D/Q110V/D146G/G185R/K433E mutant engineered for reversal in cofactor specificity for NADH over NADPH Escherichia coli
1.1.1.383 additional information recipe for altering the cofactor specificity of the ketol-acid reductoisomerases (KARIs). Combining results for an engineered NADH-dependent variant of Escherichia coli KARI with available KARI crystal structures and a comprehensive KARI-sequence alignment, key cofactor specificity determinants are identified and used to construct KARIs with reversed cofactor preference Escherichia coli
1.1.1.383 R76D/Ser78D mutant engineered for reversal in cofactor specificity for NADH over NADPH Shewanella sp.
1.1.1.383 S61D/S63D mutant engineered for reversal in cofactor specificity for NADH over NADPH Slackia exigua
1.1.1.383 S61D/S63D/I95V mutant engineered for reversal in cofactor specificity for NADH over NADPH Slackia exigua
1.1.1.383 V48L/R49P/K52E/S53D mutant engineered for reversal in cofactor specificity for NADH over NADPH Lactococcus lactis subsp. lactis
1.1.1.383 V48L/R49P/K52L/S53D mutant engineered for reversal in cofactor specificity for NADH over NADPH Lactococcus lactis subsp. lactis
1.1.1.383 V48L/R49P/K52L/S53D/E59K/T182S/E320K mutant engineered for reversal in cofactor specificity for NADH over NADPH Lactococcus lactis subsp. lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.86 0.0173
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 0.018
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.026
-
NADH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 0.027
-
NADH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.028
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.043
-
NADH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.059
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 0.08
-
NADPH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 1
-
NADPH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 1
-
NADPH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.383 0.001
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 0.001
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 0.013
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.015
-
NADH mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.026
-
NADH mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 0.03
-
NADH mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 0.041
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 0.045
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 0.047
-
NADH mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 0.075
-
NADH mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 0.09
-
NADH mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 0.108
-
NADH mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.113
-
NADH mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 0.128
-
NADH mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.285
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.415
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 0.6
-
NADPH mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 0.65
-
NADPH mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 0.75
-
NADPH mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.88
-
NADPH mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 1
-
NADPH mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 1
-
NADPH mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 1
-
NADPH mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 1.075
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 1.18
-
NADPH mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 1.4
-
NADPH mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Alicyclobacillus acidocaldarius subsp. acidocaldarius C8WR67
-
-
1.1.1.86 Methanococcus aeolicus A6UW80
-
-
1.1.1.86 Methanococcus aeolicus DSM 17508 A6UW80
-
-
1.1.1.383 Escherichia coli P05793
-
-
1.1.1.383 Lactococcus lactis subsp. lactis Q02138
-
-
1.1.1.383 Lactococcus lactis subsp. lactis IL1403 Q02138
-
-
1.1.1.383 Shewanella sp. A0KS29
-
-
1.1.1.383 Shewanella sp. ANA-3 A0KS29
-
-
1.1.1.383 Slackia exigua D0WGK0
-
-
1.1.1.383 Slackia exigua DSM 15923 D0WGK0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 (S)-2-acetolactate + NADH + H+
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.86 (S)-2-acetolactate + NADH + H+
-
Methanococcus aeolicus 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.86 (S)-2-acetolactate + NADH + H+
-
Methanococcus aeolicus DSM 17508 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.86 (S)-2-acetolactate + NADPH + H+
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
1.1.1.86 (S)-2-acetolactate + NADPH + H+
-
Methanococcus aeolicus 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
1.1.1.86 (S)-2-acetolactate + NADPH + H+
-
Methanococcus aeolicus DSM 17508 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
1.1.1.383 (S)-2-acetolactate + NADH + H+
-
Escherichia coli 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.383 (S)-2-acetolactate + NADH + H+
-
Shewanella sp. 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.383 (S)-2-acetolactate + NADH + H+
-
Slackia exigua 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.383 (S)-2-acetolactate + NADH + H+
-
Lactococcus lactis subsp. lactis 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.383 (S)-2-acetolactate + NADH + H+
-
Slackia exigua DSM 15923 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.383 (S)-2-acetolactate + NADH + H+
-
Shewanella sp. ANA-3 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.383 (S)-2-acetolactate + NADH + H+
-
Lactococcus lactis subsp. lactis IL1403 2,3-dihydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.383 (S)-2-acetolactate + NADPH + H+
-
Escherichia coli 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
1.1.1.383 (S)-2-acetolactate + NADPH + H+
-
Shewanella sp. 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
1.1.1.383 (S)-2-acetolactate + NADPH + H+
-
Slackia exigua 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
1.1.1.383 (S)-2-acetolactate + NADPH + H+
-
Lactococcus lactis subsp. lactis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
1.1.1.383 (S)-2-acetolactate + NADPH + H+
-
Slackia exigua DSM 15923 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
1.1.1.383 (S)-2-acetolactate + NADPH + H+
-
Shewanella sp. ANA-3 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?
1.1.1.383 (S)-2-acetolactate + NADPH + H+
-
Lactococcus lactis subsp. lactis IL1403 2,3-dihydroxy-3-methylbutanoate + NADP+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.86 ilvC
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 ilvC
-
Methanococcus aeolicus
1.1.1.383 ilvC
-
Slackia exigua
1.1.1.383 ilvC
-
Lactococcus lactis subsp. lactis
1.1.1.383 Shewana3_0355
-
Shewanella sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.86 0.004
-
NADPH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 0.01
-
NADPH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.013
-
NADPH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.03
-
NADH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.032
-
NADH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.15
-
NADH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 0.26
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.3
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 0.66
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.7
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.383 0.06
-
NADPH mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.08
-
NADPH mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.1
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.1
-
NADPH mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 0.1
-
NADPH mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 0.2
-
NADPH mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 0.25
-
NADPH mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 0.3
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 0.3
-
NADPH mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 0.35
-
NADH mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.35
-
NADPH mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.4
-
NADH mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.41
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 0.54
-
NADPH mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 0.8
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 0.8
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.97
-
NADH mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 1.01
-
NADH mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 1.07
-
NADH mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 1.1
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 1.3
-
NADH mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 2.3
-
NADH mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 2.4
-
NADH mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 3.6
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 4.3
-
NADH mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 4.5
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Shewanella sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.86 0.0046
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 0.0062
-
NADH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 0.01
-
NADPH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.013
-
NADPH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 0.043
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 0.05
-
NADPH mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication Methanococcus aeolicus
1.1.1.86 0.7
-
NADH mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 1.1
-
NADH mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 9
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.86 37
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Alicyclobacillus acidocaldarius subsp. acidocaldarius
1.1.1.383 0.05
-
NADPH mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.08
-
NADPH mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.11
-
NADPH mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 0.17
-
NADPH mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 0.25
-
NADPH mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 0.3
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 0.4
-
NADPH mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 0.4
-
NADPH mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 0.43
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.47
-
NADPH mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 0.5
-
NADPH mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 2.6
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 2.7
-
NADH mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 3.7
-
NADH mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 9
-
NADH wild-type, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 9
-
NADH mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 14
-
NADH mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 22
-
NADH mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 32
-
NADH mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication Shewanella sp.
1.1.1.383 65
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 71
-
NADH mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication Lactococcus lactis subsp. lactis
1.1.1.383 74
-
NADH mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 88
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 165
-
NADH mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication Escherichia coli
1.1.1.383 800
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Slackia exigua
1.1.1.383 4500
-
NADPH wild-type, pH not specified in the publication, temperature not specified in the publication Shewanella sp.