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Literature summary extracted from

  • Gangl, R.; Behmueller, R.; Tenhaken, R.
    Molecular cloning of a novel glucuronokinase/putative pyrophosphorylase from zebrafish acting in an UDP-glucuronic acid salvage pathway (2014), PLoS ONE, 9, e89690.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.43 expressed in Escherichia coli BL21(DE3) cells and Nicotiana benthamiana leaves Danio rerio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.43 0.031
-
 D-glucuronate at pH 7.5 and 35°C Danio rerio
2.7.1.43 0.059
-
 ATP at pH 7.5 and 35°C Danio rerio

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.43 Co2+ Co2+ is able to substitute nfor Mg2+ Danio rerio
2.7.1.43 Mg2+ required Danio rerio
2.7.1.43 Mn2+ Mn2+ is able to substitute nfor Mg2+ Danio rerio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.43 70000
-
 x * 70000, SDS-PAGE Danio rerio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.43 ATP + D-glucuronate Danio rerio
-
 ADP + 1-phospho-alpha-D-glucuronate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.43 Danio rerio
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.43 Protino-Ni1000 column chromatography and Strep-Tactin Macro Prep resin column chromatography Danio rerio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.43 ATP + D-glucuronate
-
 Danio rerio ADP + 1-phospho-alpha-D-glucuronate
-
 ?
2.7.1.43 additional information no activity with CTP, GTP, TTP, dATP, dCTP, dGTP and dTTP, D-glucose, D-xylose, L-arabinose, L-fucose, D-mannose, L-rhamnose, D-galactose, and D-galacturonate Danio rerio ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.43 ? x * 70000, SDS-PAGE Danio rerio

Synonyms

EC Number Synonyms Comment Organism
2.7.1.43 GKUP bifunctional enzyme glucuronokinase and putative uridyl pyrophosphorylase activities Danio rerio
2.7.1.43 glucuronokinase
-
 Danio rerio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.43 35 40 temperatures above 45°C lead to rapid inactivation of the enzyme Danio rerio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.43 7.5 8 no activity is measured below pH 4.5, whereas the maximum is reached between pH 7.5 and 8 with a far lower activity above pH 8.5 Danio rerio