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Literature summary extracted from

  • Garcia, D.E.; Keasling, J.D.
    Kinetics of phosphomevalonate kinase from Saccharomyces cerevisiae (2014), PLoS ONE, 9, e87112.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.2 expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.2 additional information the enzyme is not substrate inhibited, nor feedback inhibited by farnesyl diphosphate at concentrations up to 10 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.2 0.0743
-
 ATP at pH 7.2 and 37°C Saccharomyces cerevisiae
2.7.4.2 0.0983
-
 ATP at pH 7.2 and 30°C Saccharomyces cerevisiae
2.7.4.2 0.88
-
 (R)-5-phosphomevalonate at pH 7.2 and 37°C Saccharomyces cerevisiae
2.7.4.2 0.885
-
 (R)-5-phosphomevalonate at pH 7.2 and 30°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.2 Mg2+ dependent on, maximal activity achieved at concentrations of 10 mM or greater Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.2 ATP + (R)-5-phosphomevalonate Saccharomyces cerevisiae
-
 ADP + (R)-5-diphosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.2 Saccharomyces cerevisiae P24521
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.2 Ni-NTA resin column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.2 ATP + (R)-5-phosphomevalonate
-
 Saccharomyces cerevisiae ADP + (R)-5-diphosphomevalonate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.2 PMK
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.2 7.2
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.2 6.5 8 the enzyme activity drops off below pH 6.5 and above pH 8.0 Saccharomyces cerevisiae