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Literature summary extracted from

  • Qabazard, B.; Ahmed, S.; Li, L.; Arlt, V.M.; Moore, P.K.; Stuerzenbaum, S.R.
    C. elegans aging is modulated by hydrogen sulfide and the sulfhydrylase/cysteine synthase cysl-2 (2013), PLoS ONE, 8, e80135.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 gene cysl-2, quantitative real-time PCR enzyme expression analysis Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 additional information Caenorhabditis elegans the enzyme shows H2S synthesizing activity, cysteine synthase activity and also L-3-cyanoalanine synthase activity, EC 4.4.1.9 ?
-
 ?
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide Caenorhabditis elegans
-
 L-cysteine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Caenorhabditis elegans O45679 gene cysl-2 or K10H10.2, and gene GYY4137
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 additional information the enzyme shows H2S synthesizing activity, cysteine synthase activity and also L-3-cyanoalanine synthase activity, EC 4.4.1.9 Caenorhabditis elegans ?
-
 ?
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide
-
 Caenorhabditis elegans L-cysteine + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 CysK2
-
 Caenorhabditis elegans
2.5.1.47 CYSL
-
 Caenorhabditis elegans
2.5.1.47 cysl-2
-
 Caenorhabditis elegans
2.5.1.47 GYY4137
-
 Caenorhabditis elegans
2.5.1.47 sulfhydrylase/cysteine synthase
-
 Caenorhabditis elegans

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate
-
 Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
2.5.1.47 malfunction cysl-2 mutant phenotype, overview. The cysl-2 mutant has a reduced rate of enzymatic H2S synthesis from the added substrates L-cysteine and is marked by a decline in reproductive output compared to wild-type worms. Body area and length are significantly lower in cysl-2 compared to age-matched wild type worms, and GYY4137 treatment effectively rescues this phenotype. Lifespan is reduced in cysl-2 compared to wild-type and the addition of GYY4137 reverses this effect and increases the median survival of cysl-2 Caenorhabditis elegans
2.5.1.47 physiological function the bifunctional enzyme, encoded by gene K10H10.2, or cysl-2, shows cysteine synthase activity and also L-3-cyanoalanine synthase activity, EC 4.4.1.9. It has been described as a hypoxia inducible factor (HIF) target. The enzyme regulates the mRNA expression of aging-associated and stress-related genes in wild-type Caenorhabditis elegans. Enzyme treatment protects the wild-type nematodes against oxidative, endoplasmic reticulum stress, and thermal stressors but not cadmium-induced metallic stress. The enzyme is active in vitro and in vivo and protects against lipopolysaccharide-induced inflammation. GYY4137 induces longevity and stress resistance, mechanism of action via induction of T24B8.5 and altered transcriptional control of SKN-1 Caenorhabditis elegans