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Literature summary extracted from

  • Issur, M.; Bougie, I.; Despins, S.; Bisaillon, M.
    Enzymatic synthesis of RNAs capped with nucleotide analogues reveals the molecular basis for substrate selectivity of RNA capping enzyme: impacts on RNA metabolism (2013), PLoS ONE, 8, e75310.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.50 2'-fluoro-2'-deoxy-GTP
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 2-amino-6-chloropurine-ribose triphosphate
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 3'-deoxy-GTP
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 3'-O-Methyl-GTP
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 6-methylthio-GTP
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 6-thio-GTP
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 8-bromo-GTP
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 8-iodoGTP
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 GTP
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 ITP
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 N1-methyl-GTP
-
 Paramecium bursaria Chlorella virus 1
2.7.7.50 O6-methyl-GTP
-
 Paramecium bursaria Chlorella virus 1

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.50 Paramecium bursaria Chlorella virus 1 Q84424
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.50 2'-fluoro-2'-deoxy-GTP + pp(5')RNA 0.16fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 2'-fluoro-2'-deoxy-G(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 2-amino-6-chloropurine-beta-D-ribose triphosphate + pp(5')RNA 0.08fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 2-amino-6-chloropurine-beta-D-ribose-(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 3'-deoxy-GTP + pp(5')RNA 0.15fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 3'-deoxy-G(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 3'-O-methyl-GTP + pp(5')RNA 0.38fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 3'-O-methyl-G(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 6-methylthio-GTP + pp(5')RNA 0.07fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 6-methylthio-G(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 6-thio-GTP + pp(5')RNA 0.16fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 6-thio-G(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 8-bromo-GTP + pp(5')RNA 0.40fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 8-bromo-G(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 8-iodoGTP + pp(5')RNA 1.0fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 8-iodo-G(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 GTP + pp(5')RNA
-
 Paramecium bursaria Chlorella virus 1 G(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 ITP + pp(5')RNA 1.3fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 I(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 additional information generation of RNAs harboring various different cap analogues. Cap-dependent translation can occur in the absence of theN7-methyl group on the cap structure provided that alternative modifications enable appropriate binding to eIF4E Paramecium bursaria Chlorella virus 1 ?
-
 ?
2.7.7.50 N1-methyl-GTP + pp(5')RNA 1.1fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 N1-methyl-G(5')ppp(5')RNA + diphosphate
-
 ?
2.7.7.50 O6-methyl-GTP + pp(5')RNA 0.48fold efficiency compared with substrate GTP Paramecium bursaria Chlorella virus 1 O6-methyl-G(5')ppp(5')RNA + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.50 A103R
-
 Paramecium bursaria Chlorella virus 1

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.50 0.08
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 N1-methyl-GTP
2.7.7.50 0.1
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 GTP
2.7.7.50 0.15
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 O6-methyl-GTP
2.7.7.50 0.15
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 3'-deoxy-GTP
2.7.7.50 0.16
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 2-amino-6-chloropurine-ribose triphosphate
2.7.7.50 0.22
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 6-thio-GTP
2.7.7.50 0.34
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 ITP
2.7.7.50 0.42
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 8-iodoGTP
2.7.7.50 0.43
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 6-methylthio-GTP
2.7.7.50 0.45
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 3'-O-Methyl-GTP
2.7.7.50 1.4
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 2'-fluoro-2'-deoxy-GTP
2.7.7.50 1.5
-
 IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C Paramecium bursaria Chlorella virus 1 8-bromo-GTP