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Literature summary extracted from

  • Cheng, C.S.; Jia, K.F.; Chen, T.; Chang, S.Y.; Lin, M.S.; Yin, H.S.
    Experimentally validated novel inhibitors of Helicobacter pylori phosphopantetheine adenylyltransferase discovered by virtual high-throughput screening (2013), PLoS ONE, 8, e74271.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.3 drug development the enzyme is an attractive target for antibacterial drug development against Helicobacter pylori Helicobacter pylori
2.7.7.3 medicine the enzyme is a therapeutical target in treatment of Helicobacter induced gastritis Helicobacter pylori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.3 recombinant expression in Escherichia coli strain BL21(DE3) Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.3 AC1LDVWJ
-
 Helicobacter pylori
2.7.7.3 D-amethopterin a mixed-type inhibitor of HpPPAT activity, that simultaneously occupies the HpPPAT 4'-phosphopantetheine- and ATP-binding sites, the compound can serve as a lead compound in drug development, steady-state kinetic inhibition assay. Model of HpPPAT complexed with D-amethopterin, overview Helicobacter pylori
2.7.7.3 additional information inhibitor virtual high-throughput screening using the HpPPAT crystal structure, docking study, overview Helicobacter pylori
2.7.7.3 Omeprazole
-
 Helicobacter pylori
2.7.7.3 T5568746
-
 Helicobacter pylori
2.7.7.3 zanamivir
-
 Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.3 Mg2+ required Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.3 ATP + pantetheine 4'-phosphate Helicobacter pylori
-
 diphosphate + 3'-dephospho-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.3 Helicobacter pylori O26010
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Helicobacter pylori diphosphate + 3'-dephospho-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.3 phosphopantetheine adenylyltransferase
-
 Helicobacter pylori
2.7.7.3 PPAT
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.3 25
-
 assay at Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.3 7.9
-
 assay at Helicobacter pylori

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.3 0.2674
-
 D-amethopterin pH 7.9, 25°C, recombinant enzyme Helicobacter pylori

General Information

EC Number General Information Comment Organism
2.7.7.3 malfunction enzyme inactivation effectively prevents bacterial viability Helicobacter pylori
2.7.7.3 metabolism the enzyme catalyzes the penultimate step in coenzyme A biosynthesis Helicobacter pylori