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Literature summary extracted from

  • Zhou, S.; Mohan Raj, S.; Ashok, S.; Edwardraja, S.; Lee, S.G.; Park, S.
    Cloning, expression and characterization of 3-hydroxyisobutyrate dehydrogenase from Pseudomonas denitrificans ATCC 13867 (2013), PLoS ONE, 8, e62666.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.31 2-mercaptoethanol about 182.8% activity at 1 mM Pseudomonas denitrificans (nom. rej.)
1.1.1.31 dithiothreitol about 184.8% activity at 1 mM Pseudomonas denitrificans (nom. rej.)
1.1.1.31 EDTA about 150.9% activity at 1 mM Pseudomonas denitrificans (nom. rej.)

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.31 expressed in Escherichia coli BL21(DE3) cells Pseudomonas denitrificans (nom. rej.)

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.31 Ag+ complete inhibition at 1 mM Pseudomonas denitrificans (nom. rej.)
1.1.1.31 Co2+ about 64.3% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
1.1.1.31 Cu2+ about 14.5% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
1.1.1.31 Fe2+ about 9.1% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
1.1.1.31 Hg2+ complete inhibition at 1 mM Pseudomonas denitrificans (nom. rej.)
1.1.1.31 Mg2+ about 88.6% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
1.1.1.31 Ni2+ about 62.3% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)
1.1.1.31 Zn2+ about 14.5% residual activity at 1 mM Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.31 0.164
-
 NAD+ with 3-hydroxypropionic acid as cosubstrate, at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 0.178
-
 (S)-3-hydroxyisobutyrate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 1.055
-
 3-hydroxypropionic acid at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 24.18
-
 methyl DL-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 25.14
-
 methyl (2S)-3-hydroxy-2-methylpropanoate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 117.5
-
 L-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.31 Mn2+ about 130.7% activity at 1 mM Pseudomonas denitrificans (nom. rej.)
1.1.1.31 additional information not influenced by Na+, K+, NH3+, and Li+ Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.31 30000
-
 x * 30000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.31 Pseudomonas denitrificans (nom. rej.)
-
-
-
1.1.1.31 Pseudomonas denitrificans (nom. rej.) ATCC 13867
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.31 Ni-NTA resin column chromatography Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is very specific to (S)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
1.1.1.31 (S)-3-hydroxyisobutyrate + NAD+ the enzyme is very specific to (S)-3-hydroxyisobutyrate Pseudomonas denitrificans (nom. rej.) ATCC 13867 2-methyl-3-oxopropanoate + NADH + H+
-
 ?
1.1.1.31 3-hydroxypropionic acid + NAD+
-
 Pseudomonas denitrificans (nom. rej.) malonate semialdehyde + NADH + H+
-
 r
1.1.1.31 3-hydroxypropionic acid + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 malonate semialdehyde + NADH + H+
-
 r
1.1.1.31 L-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) 3-oxo-L-alanine + NADH + H+
-
 r
1.1.1.31 L-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 3-oxo-L-alanine + NADH + H+
-
 r
1.1.1.31 methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
 Pseudomonas denitrificans (nom. rej.) methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
 r
1.1.1.31 methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
 r
1.1.1.31 methyl DL-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) methyl 3-oxo-DL-alanine + NADH + H+
-
 r
1.1.1.31 methyl DL-serine + NAD+
-
 Pseudomonas denitrificans (nom. rej.) ATCC 13867 methyl 3-oxo-DL-alanine + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.31 ? x * 30000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Synonyms

EC Number Synonyms Comment Organism
1.1.1.31 3HIBDH-IV
-
 Pseudomonas denitrificans (nom. rej.)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.31 30
-
-
 Pseudomonas denitrificans (nom. rej.)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.31 25 40 more than 80% activity between 25°C and 40°C Pseudomonas denitrificans (nom. rej.)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.31 6.62
-
 methyl DL-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 6.717
-
 NAD+ with 3-hydroxypropionic acid as cosubstrate, at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 10.8
-
 3-hydroxypropionic acid at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 35.76
-
 methyl (2S)-3-hydroxy-2-methylpropanoate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 47.55
-
 L-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 80.79
-
 (S)-3-hydroxyisobutyrate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.31 9
-
-
 Pseudomonas denitrificans (nom. rej.)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.31 8 10 more than 60% activity between pH 8.0 and 10.0 Pseudomonas denitrificans (nom. rej.)

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.31 NAD+ dependent on Pseudomonas denitrificans (nom. rej.)

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.31 0.275
-
 methyl DL-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 0.404
-
 L-serine at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 1.433
-
 methyl (2S)-3-hydroxy-2-methylpropanoate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 10.23
-
 3-hydroxypropionic acid at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 42.44
-
 NAD+ with 3-hydroxypropionic acid as cosubstrate, at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)
1.1.1.31 464.9
-
 (S)-3-hydroxyisobutyrate at 30°C and pH 9.0 Pseudomonas denitrificans (nom. rej.)