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Literature summary extracted from

  • Bullard-Feibelman, K.M.; Fuller, B.P.; Geiss, B.J.
    A sensitive and robust high-throughput screening assay for inhibitors of the Chikungunya virus nsP1 capping enzyme (2016), PLoS ONE, 11, e0158923.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.50 analysis fluorescence polarization-based assay that monitors displacement of a fluorescently-labeled GTP analog in real time and a secondary orthogonal assay that measures guanylation activity. This platform provides an avenue for identification of potent nsP1 inhibitors Chikungunya virus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.50 expression in Escherichia coli Chikungunya virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.50 7-methyl-GTP
-
 Chikungunya virus
2.7.7.50 ATP
-
 Chikungunya virus
2.7.7.50 ddGTP
-
 Chikungunya virus
2.7.7.50 GpppA
-
 Chikungunya virus
2.7.7.50 GTP
-
 Chikungunya virus
2.7.7.50 ITP
-
 Chikungunya virus
2.7.7.50 XTP
-
 Chikungunya virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.50 61000
-
-
 Chikungunya virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.50 Chikungunya virus Q1H8W7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.50 8-[(6-amino)hexyl]-amino-GTP-ATTO-680 + pp(5')RNA fluorescent substrate analog Chikungunya virus G(5')ppp(5')RNA + diphosphate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.50 ? x * 61000, SDS-PAGE, x * 61088, mass spectrometry, recombinant protein with His-tag Chikungunya virus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.50 nsP1
-
 Chikungunya virus