EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.3.9 | medicine | the enzyme can function as an adhesin, evaluation of its potential as a vaccine. Immunization with rPtsA protects mice against a sublethal intranasal and a lethal intravenous pneumococcal challenge. PtsA binding peptides derived from its putative target molecules can be considered for development of therapeutics, and rPtsA is a candidate for vaccine development | Streptococcus pneumoniae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.3.9 | gene ptsI, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, recombinant expression in Escherichia coli strain BL21(DE3)pLysS | Streptococcus pneumoniae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.3.9 | additional information | enzyme PtsA binding peptides derived from its putative target molecules, i.e. AVVVNDANRTRK, ADDESPEMKPGX, TXQVSNHKNHTN, LDSIRIIVIRRI, ADGGVPV, and DQRPAXRRMYRA, inhibit pneumococcal adhesion in vitro to lung- and tracheal-derived cell lines. In addition, the tested peptides inhibit lung colonization after intranasal inoculation of mice with Streptococcus pneumoniae | Streptococcus pneumoniae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.3.9 | cell wall | - |
Streptococcus pneumoniae | 5618 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.3.9 | 120100 | - |
recombinant untagged enzyme, mass spectrometry | Streptococcus pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.3.9 | additional information | Streptococcus pneumoniae | the enzyme can function as an adhesin, identification of host target molecules, overview | ? | - |
? | |
2.7.3.9 | phosphoenolpyruvate + protein histidine | Streptococcus pneumoniae | - |
pyruvate + protein Npi-phospho-L-histidine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.3.9 | Streptococcus pneumoniae | A0A0H2ZMY1 | serotype 2 strain D39 and strain WU2, and the unencapsulated derivative of strain WU2, namely strain 3.8DW, and the unencapsulated derivative of strain D39, namely strain R6, gene ptsI | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.3.9 | additional information | the enzyme can function as an adhesin, identification of host target molecules, overview | Streptococcus pneumoniae | ? | - |
? | |
2.7.3.9 | phosphoenolpyruvate + protein histidine | - |
Streptococcus pneumoniae | pyruvate + protein Npi-phospho-L-histidine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.3.9 | dimer | 2 * 63165,sequence calculation, 2 * 70000-72000, recombinant untagged enzyme, SDS-PAGE, 2 * 62492-63932, recombinant untagged enzyme, mass spectrometry | Streptococcus pneumoniae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.3.9 | phosphoenolpyruvate protein phosphotransferase | - |
Streptococcus pneumoniae |
2.7.3.9 | ptsA | - |
Streptococcus pneumoniae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.3.9 | physiological function | recombinant PtsA and anti-rPtsA antiserum inhibit adhesion of Streptococcus pneumoniae to cultured human lung adenocarcinoma A549 cells, epitope screening, overview. Homologous sequences are found in human BMPER, multimerin1, protocadherin19, integrinbeta4, epsin1 and collagen type VIIalpha1 proteins, all of which can be found in A549 cells except the latter. anti-rPtsA Antiserum reduces bacterial virulence in the intravenous inoculation mouse model. The surface-localized PtsA functions as an adhesin | Streptococcus pneumoniae |