EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.8.7 | drug development | the enzyme is a broad-spectrum target for design of antifungal drugs | Candida albicans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.7 | gene PPT2, DNA and amino acid sequence determination and analysis, recombinant expression of tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) | Candida albicans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.8.7 | additional information | cloning of PPT2 promoter replacement construct and a deletion construct from strain SN76, by placing one allele under the control of the regulatable MET3 promoter, and deleting the remaining allele. Phenotypes of the heterozygous and the conditional null mutant, overview | Candida albicans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.7 | Mg2+ | dependent on | Candida albicans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.7 | CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Candida albicans | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.7 | Candida albicans | Q5APF3 | strain ATCC MYA-2876 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.8.7 | recombinant tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography | Candida albicans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.7 | CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Candida albicans | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
2.7.8.7 | CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | catalytic activity of Ppt2 as a phosphopantetheinyl transferase and the acyl carrier protein Acp1 as a substrate, Acp12 is no substrate. The terminal thiol group of the 4'PPT is the site at which elongation occurs via thioester linkages and attachments are covalently linked | Candida albicans | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.8.7 | C1_09480W | - |
Candida albicans |
2.7.8.7 | phosphopantetheinyl transferase | - |
Candida albicans |
2.7.8.7 | PPT2 | - |
Candida albicans |
2.7.8.7 | Ppt2p | - |
Candida albicans |
2.7.8.7 | PPTase | - |
Candida albicans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.8.7 | 22 | - |
assay at room temperature | Candida albicans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.8.7 | 7.5 | - |
assay at | Candida albicans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.8.7 | physiological function | The 4'-phosphopantetheine (4'PPT) portion of coenzyme A (CoA) is an essential group for many carrier proteins and enzymes. Addition of this group is required for the correct function of polyketide synthase (PKS), non-ribosomal peptide synthetase and fatty acid synthase. The 4'PPT group is transferred to a highly conserved serine motif in the acceptor protein in a magnesium dependent reaction by phosphopantetheinyl transferases (PPTases). Gene PPT2 encoding a phosphopantetheinyl transferase and is essential for growth in Candida albicans | Candida albicans |