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Literature summary extracted from

  • Dobb, K.S.; Kaye, S.J.; Beckmann, N.; Thain, J.L.; Stateva, L.; Birch, M.; Oliver, J.D.
    Characterisation of the Candida albicans phosphopantetheinyl transferase Ppt2 as a potential antifungal drug target (2015), PLoS ONE, 10, e0143770.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.8.7 drug development the enzyme is a broad-spectrum target for design of antifungal drugs Candida albicans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 gene PPT2, DNA and amino acid sequence determination and analysis, recombinant expression of tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.7 additional information cloning of PPT2 promoter replacement construct and a deletion construct from strain SN76, by placing one allele under the control of the regulatable MET3 promoter, and deleting the remaining allele. Phenotypes of the heterozygous and the conditional null mutant, overview Candida albicans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.7 Mg2+ dependent on Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] Candida albicans
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Candida albicans Q5APF3 strain ATCC MYA-2876
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 recombinant tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
Candida albicans adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] catalytic activity of Ppt2 as a phosphopantetheinyl transferase and the acyl carrier protein Acp1 as a substrate, Acp12 is no substrate. The terminal thiol group of the 4'PPT is the site at which elongation occurs via thioester linkages and attachments are covalently linked Candida albicans adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 C1_09480W
-
Candida albicans
2.7.8.7 phosphopantetheinyl transferase
-
Candida albicans
2.7.8.7 PPT2
-
Candida albicans
2.7.8.7 Ppt2p
-
Candida albicans
2.7.8.7 PPTase
-
Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.7 22
-
assay at room temperature Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.7 7.5
-
assay at Candida albicans

General Information

EC Number General Information Comment Organism
2.7.8.7 physiological function The 4'-phosphopantetheine (4'PPT) portion of coenzyme A (CoA) is an essential group for many carrier proteins and enzymes. Addition of this group is required for the correct function of polyketide synthase (PKS), non-ribosomal peptide synthetase and fatty acid synthase. The 4'PPT group is transferred to a highly conserved serine motif in the acceptor protein in a magnesium dependent reaction by phosphopantetheinyl transferases (PPTases). Gene PPT2 encoding a phosphopantetheinyl transferase and is essential for growth in Candida albicans Candida albicans