EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.4.42 | structure of AtzC at 1.84 A with weak inhibitor malonate bound in the active site. Malonate adopts a planar conformation in the structure and packs against residue as well as interacting with Lys65, Gln160, His219 and Asn304. Enzyme is a dimer of dimers | Pseudomonas sp. ADP |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.4.42 | D188A | about 6fold increase in kcat/Km value | Pseudomonas sp. ADP |
3.5.4.42 | D188N | activity similar to wild-type | Pseudomonas sp. ADP |
3.5.4.42 | D303A | activity similar to wild-type | Pseudomonas sp. ADP |
3.5.4.42 | D303N | activity similar to wild-type | Pseudomonas sp. ADP |
3.5.4.42 | H219A | 4fold increase in kcat value | Pseudomonas sp. ADP |
3.5.4.42 | H249A | substitution of an active site histidine residue, complete loss of activity | Pseudomonas sp. ADP |
3.5.4.42 | K65A | more than 15fold increase in kcat/Km value, 30fold increase in kcat for ammelide | Pseudomonas sp. ADP |
3.5.4.42 | K65R | more than 15fold increase in kcat/Km value, 12fold increase in kcat for ammelide | Pseudomonas sp. ADP |
3.5.4.42 | N304A | slight increase in kcat for ammelide | Pseudomonas sp. ADP |
3.5.4.42 | N304D | 7fold increase in kcat for ammelide | Pseudomonas sp. ADP |
3.5.4.42 | Q160A | activity similar to wild-type | Pseudomonas sp. ADP |
3.5.4.42 | Q160E | 60% reduction in kcat value | Pseudomonas sp. ADP |
3.5.4.42 | W309A | mutation opens up the area above the ring and increases the catalytic rate by almost a factor of two | Pseudomonas sp. ADP |
3.5.4.42 | W309F | slight reduction in catalytic rate | Pseudomonas sp. ADP |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.42 | 1.3 | - |
ammelide | mutant Q160R, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 1.6 | - |
ammelide | mutant H219A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 1.7 | - |
ammelide | mutant D303A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 1.8 | - |
ammelide | mutant Q160A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 1.9 | - |
ammelide | mutant D188N, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 2.2 | - |
ammelide | mutant D188A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 2.4 | - |
ammelide | mutant K65R, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 3 | - |
ammelide | mutant W309F, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 3.1 | - |
ammelide | wild-type, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 4.2 | - |
ammelide | mutant N304A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 5.6 | - |
ammelide | mutant W309A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 6.1 | - |
ammelide | mutant D303N, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 6.1 | - |
ammelide | mutant K65A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 25 | - |
ammelide | mutant N304D, pH 7.5, 28°C | Pseudomonas sp. ADP |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.42 | Pseudomonas sp. ADP | O52063 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.42 | ammelide + H2O | - |
Pseudomonas sp. ADP | cyanuric acid + ammonia | - |
? | |
3.5.4.42 | N-isopropylammelide + H2O | - |
Pseudomonas sp. ADP | cyanuric acid + isopropylamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.4.42 | tetramer | crystallization data | Pseudomonas sp. ADP |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.4.42 | AtzC | - |
Pseudomonas sp. ADP |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.4.42 | 57 | - |
melting temperature, mutant H249A | Pseudomonas sp. ADP |
3.5.4.42 | 60 | - |
melting temperature, wild-type | Pseudomonas sp. ADP |
3.5.4.42 | 63 | - |
melting temperature, mutant H219A | Pseudomonas sp. ADP |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.42 | 10.4 | - |
ammelide | mutant Q160R, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 14.1 | - |
ammelide | mutant D188N, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 16.8 | - |
ammelide | mutant H219A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 21.3 | - |
ammelide | mutant W309F, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 23 | - |
ammelide | mutant Q160A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 26.2 | - |
ammelide | wild-type, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 29.3 | - |
ammelide | mutant N304A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 35.8 | - |
ammelide | mutant D303A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 42.4 | - |
ammelide | mutant W309A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 90.4 | - |
ammelide | mutant D303N, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 106.5 | - |
ammelide | mutant D188A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 189 | - |
ammelide | mutant N304D, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 332.2 | - |
ammelide | mutant K65R, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 778.5 | - |
ammelide | mutant K65A, pH 7.5, 28°C | Pseudomonas sp. ADP |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.42 | 7 | - |
ammelide | mutant N304A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 7.1 | - |
ammelide | mutant W309F, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 7.4 | - |
ammelide | mutant D188N, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 7.6 | - |
ammelide | mutant N304D, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 7.6 | - |
ammelide | mutant W309A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 8.1 | - |
ammelide | mutant Q160R, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 8.5 | - |
ammelide | wild-type, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 11 | - |
ammelide | mutant H219A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 13 | - |
ammelide | mutant Q160A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 15 | - |
ammelide | mutant D303N, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 21 | - |
ammelide | mutant D303A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 49 | - |
ammelide | mutant D188A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 130 | - |
ammelide | mutant K65A, pH 7.5, 28°C | Pseudomonas sp. ADP | |
3.5.4.42 | 140 | - |
ammelide | mutant K65R, pH 7.5, 28°C | Pseudomonas sp. ADP |