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Literature summary extracted from

  • Yin, X.; Hu, D.; Li, J.; He, Y.; Zhu, T.; Wu, M.
    Contribution of disulfide bridges to the thermostability of a type a feruloyl esterase from Aspergillus usamii (2015), PLoS ONE, 10, e0126864.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Pichia pastoris Aspergillus usamii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 A126C/N152C introduction of a disulfide bridge, increase of temperature optimum by 6°C and increase in thermal inactivation half-live Aspergillus usamii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 3.62
-
 ferulic acid 4-nitrophenyl ester wild-type, pH 5.5, 40°C Aspergillus usamii
3.1.1.73 5.98
-
 ferulic acid 4-nitrophenyl ester mutant A126C/N152C, pH 5.5, 40°C Aspergillus usamii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 36000
-
 x * 36000, SDS-PAGE Aspergillus usamii

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus usamii V5TEY2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ferulic acid 4-nitrophenyl ester + H2O
-
 Aspergillus usamii ferulic acid + 4-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 36000, SDS-PAGE Aspergillus usamii

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAEA
-
 Aspergillus usamii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 45
-
 wild-type Aspergillus usamii
3.1.1.73 51
-
 mutant A126C/N152C Aspergillus usamii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 55
-
 half-life of wild-type 15 min, of mutant A126C/N152C 188 min, respectively Aspergillus usamii
3.1.1.73 60
-
 half-life of wild-type 4 min, of mutant A126C/N152C 40 min, respectively Aspergillus usamii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 135
-
 ferulic acid 4-nitrophenyl ester wild-type, pH 5.5, 40°C Aspergillus usamii
3.1.1.73 213
-
 ferulic acid 4-nitrophenyl ester mutant A126C/N152C, pH 5.5, 40°C Aspergillus usamii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 35.68
-
 ferulic acid 4-nitrophenyl ester mutant A126C/N152C, pH 5.5, 40°C Aspergillus usamii
3.1.1.73 37.25
-
 ferulic acid 4-nitrophenyl ester wild-type, pH 5.5, 40°C Aspergillus usamii