Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Poyraz, O.e.; Brunner, K.; Lohkamp, B.; Axelsson, H.; Hammarstroem, L.G.; Schnell, R.; Schneider, G.
    Crystal structures of the kinase domain of the sulfate-activating complex in Mycobacterium tuberculosis (2015), PLoS ONE, 10, e0121494.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.25 dithiothreitol the enzyme activity is about 3fold stimulated in the presence of 2 mM dithiothreitol Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.25 in complex with ligands, hanging drop vapor diffusion method, using 0.2 M NH4NO3, 20% (w/v) PEG3350 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.25 C541A the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
2.7.1.25 C549A the mutant shows increased activity compared to the wild type enzyme Mycobacterium tuberculosis
2.7.1.25 C556S inactive Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 42000
-
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.25 ATP + adenosine-5'-phosphosulfate Mycobacterium tuberculosis
-
 ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Mycobacterium tuberculosis P9WNM5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 Ni-NTA resin column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine-5'-phosphosulfate
-
 Mycobacterium tuberculosis ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 homodimer 2 * 21260, electrospray ionization mass spectrometry Mycobacterium tuberculosis
2.7.1.25 homodimer 2 * 21261, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.25 CysC
-
 Mycobacterium tuberculosis