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Literature summary extracted from

  • Zhang, Z.; Zheng, Y.; Petukh, M.; Pegg, A.; Ikeguchi, Y.; Alexov, E.
    Enhancing human spermine synthase activity by engineered mutations (2013), PLoS Comput. Biol., 9, e1002924.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.22 expressed in Escherichia coli XL-1 Blue cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.22 S165D/L175E/T178H/C206R the mutant shows increased activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.22 0.0005
-
S-adenosyl 3-(methylthio)propylamine wild type enzyme, at pH 7.5 and 37°C Homo sapiens
2.5.1.22 0.0009
-
S-adenosyl 3-(methylthio)propylamine mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C Homo sapiens
2.5.1.22 0.6
-
spermidine mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C Homo sapiens
2.5.1.22 0.8
-
spermidine wild type enzyme, at pH 7.5 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.22 S-adenosyl 3-(methylthio)propylamine + spermidine Homo sapiens
-
S-methyl-5'-thioadenosine + spermine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.22 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.22 TALON affinity resin column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.22 227
-
mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C Homo sapiens
2.5.1.22 2425
-
wild type enzyme, at pH 7.5 and 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.22 S-adenosyl 3-(methylthio)propylamine + spermidine
-
Homo sapiens S-methyl-5'-thioadenosine + spermine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.22 SMS
-
Homo sapiens