EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.22 | expressed in Escherichia coli XL-1 Blue cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.22 | S165D/L175E/T178H/C206R | the mutant shows increased activity compared to the wild type enzyme | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.22 | 0.0005 | - |
S-adenosyl 3-(methylthio)propylamine | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
2.5.1.22 | 0.0009 | - |
S-adenosyl 3-(methylthio)propylamine | mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C | Homo sapiens | |
2.5.1.22 | 0.6 | - |
spermidine | mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C | Homo sapiens | |
2.5.1.22 | 0.8 | - |
spermidine | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.22 | S-adenosyl 3-(methylthio)propylamine + spermidine | Homo sapiens | - |
S-methyl-5'-thioadenosine + spermine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.22 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.22 | TALON affinity resin column chromatography | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.22 | 227 | - |
mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C | Homo sapiens |
2.5.1.22 | 2425 | - |
wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.22 | S-adenosyl 3-(methylthio)propylamine + spermidine | - |
Homo sapiens | S-methyl-5'-thioadenosine + spermine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.22 | SMS | - |
Homo sapiens |