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Literature summary extracted from

  • Saigo, M.; Alvarez, C.; Andreo, C.; Drincovich, M.
    Plastidial NADP-malic enzymes from grasses: Unraveling the way to the C4 specific isoforms (2013), Plant Physiol. Biochem., 63, 39-48.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.40 expressed in Escherichia coli BL21 cells Zea mays
1.1.1.40 expressed in Escherichia coli BL21 cells Sorghum bicolor
1.1.1.40 expressed in Escherichia coli BL21 cells Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.40 malate the enzyme is inhibited by high malate concentration at pH 7.0 Oryza sativa
1.1.1.40 malate the enzyme is inhibited by high malate concentration at pH 7.0 Sorghum bicolor
1.1.1.40 malate the enzyme is inhibited by high malate concentration at pH 7.0 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 0.008
-
NADP+ at pH 8.0, temperature not specified in the publication Zea mays
1.1.1.40 0.011
-
NADP+ at pH 8.0, temperature not specified in the publication Sorghum bicolor
1.1.1.40 0.035
-
NADP+ at pH 8.0, temperature not specified in the publication Oryza sativa
1.1.1.40 0.19
-
(S)-malate at pH 8.0, temperature not specified in the publication Zea mays
1.1.1.40 0.36
-
(S)-malate at pH 8.0, temperature not specified in the publication Sorghum bicolor
1.1.1.40 0.81
-
(S)-malate at pH 8.0, temperature not specified in the publication Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.40 plastid
-
Zea mays 9536
-
1.1.1.40 plastid
-
Sorghum bicolor 9536
-
1.1.1.40 plastid
-
Oryza sativa 9536
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ Zea mays
-
pyruvate + CO2 + NADPH
-
?
1.1.1.40 (S)-malate + NADP+ Sorghum bicolor
-
pyruvate + CO2 + NADPH
-
?
1.1.1.40 (S)-malate + NADP+ Oryza sativa
-
pyruvate + CO2 + NADPH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Oryza sativa
-
-
-
1.1.1.40 Sorghum bicolor
-
-
-
1.1.1.40 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40 Ni-NTA column chromatography Zea mays
1.1.1.40 Ni-NTA column chromatography Sorghum bicolor
1.1.1.40 Ni-NTA column chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.40 leaf
-
Zea mays
-
1.1.1.40 leaf
-
Sorghum bicolor
-
1.1.1.40 leaf
-
Oryza sativa
-
1.1.1.40 root
-
Zea mays
-
1.1.1.40 root
-
Sorghum bicolor
-
1.1.1.40 root
-
Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+
-
Zea mays pyruvate + CO2 + NADPH
-
?
1.1.1.40 (S)-malate + NADP+
-
Sorghum bicolor pyruvate + CO2 + NADPH
-
?
1.1.1.40 (S)-malate + NADP+
-
Oryza sativa pyruvate + CO2 + NADPH
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.40 L-malate:NADP oxidoreductase
-
Zea mays
1.1.1.40 L-malate:NADP oxidoreductase
-
Sorghum bicolor
1.1.1.40 L-malate:NADP oxidoreductase
-
Oryza sativa
1.1.1.40 NADP-ME
-
Zea mays
1.1.1.40 NADP-ME
-
Sorghum bicolor
1.1.1.40 NADP-ME
-
Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.40 181.6
-
(S)-malate at pH 8.0, temperature not specified in the publication Oryza sativa
1.1.1.40 198.9
-
(S)-malate at pH 8.0, temperature not specified in the publication Zea mays
1.1.1.40 275.7
-
(S)-malate at pH 8.0, temperature not specified in the publication Sorghum bicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.40 NADP+
-
Zea mays
1.1.1.40 NADP+
-
Sorghum bicolor
1.1.1.40 NADP+
-
Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.40 224.1
-
(S)-malate at pH 8.0, temperature not specified in the publication Oryza sativa
1.1.1.40 765.7
-
(S)-malate at pH 8.0, temperature not specified in the publication Sorghum bicolor
1.1.1.40 1047
-
(S)-malate at pH 8.0, temperature not specified in the publication Zea mays
1.1.1.40 5800
-
NADP+ at pH 8.0, temperature not specified in the publication Oryza sativa
1.1.1.40 27900
-
NADP+ at pH 8.0, temperature not specified in the publication Sorghum bicolor
1.1.1.40 61200
-
NADP+ at pH 8.0, temperature not specified in the publication Zea mays