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Literature summary extracted from

  • Qin, H.; Deng, Z.; Zhang, C.; Wang, Y.; Wang, J.; Liu, H.; Zhang, Z.; Huang, R.; Zhang, Z.
    Rice GDP-mannose pyrophosphorylase OsVTC1-1 and OsVTC1-3 play different roles in ascorbic acid synthesis (2016), Plant Mol. Biol., 90, 317-327.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 gene OsVTC1-1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Oryza sativa Japonica Group
2.7.7.13 gene OsVTC1-3, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Oryza sativa Japonica Group
2.7.7.13 gene OsVTC1-8, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.13 additional information generation of OsVTC1-1 silenced mutant plants by RNAi method Oryza sativa Japonica Group
2.7.7.13 additional information generation of OsVTC1-3 silenced mutant plants by RNAi method Oryza sativa Japonica Group
2.7.7.13 additional information generation of OsVTC1-8 silenced mutant plants by RNAi method Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Mg2+ required Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Oryza sativa Japonica Group
-
diphosphate + GDP-mannose
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Oryza sativa Japonica Group Q6Z9A3 OsVTC1-8 or GMPP3
-
2.7.7.13 Oryza sativa Japonica Group Q84JH5
-
-
2.7.7.13 Oryza sativa Japonica Group Q84JH5 OsVTC1-3 or GMPP1
-
2.7.7.13 Oryza sativa Japonica Group Q941T9
-
-
2.7.7.13 Oryza sativa Japonica Group Q941T9 OsVTC1-1 or GMPP2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.13 leaf
-
Oryza sativa Japonica Group
-
2.7.7.13 leaf expression is high in leaf Oryza sativa Japonica Group
-
2.7.7.13 leaf high light-induced expression level Oryza sativa Japonica Group
-
2.7.7.13 additional information OsVTC1-8 transcript level is low in both leaves and roots Oryza sativa Japonica Group
-
2.7.7.13 root
-
Oryza sativa Japonica Group
-
2.7.7.13 root expression is high in root Oryza sativa Japonica Group
-
2.7.7.13 root high dark-induced expression level Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
Oryza sativa Japonica Group diphosphate + GDP-mannose
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.13 GDP-D-mannose pyrophosphorylase
-
Oryza sativa Japonica Group
2.7.7.13 GDP-mannose pyrophosphorylase
-
Oryza sativa Japonica Group
2.7.7.13 GMPase
-
Oryza sativa Japonica Group
2.7.7.13 GMPP1
-
Oryza sativa Japonica Group
2.7.7.13 GMPP2
-
Oryza sativa Japonica Group
2.7.7.13 GMPP3
-
Oryza sativa Japonica Group
2.7.7.13 Os01g0847200
-
Oryza sativa Japonica Group
2.7.7.13 Os03g0268400
-
Oryza sativa Japonica Group
2.7.7.13 OsVTC1-1
-
Oryza sativa Japonica Group
2.7.7.13 OsVTC1-3
-
Oryza sativa Japonica Group
2.7.7.13 OsVTC1-8
-
Oryza sativa Japonica Group
2.7.7.13 VCT1-1
-
Oryza sativa Japonica Group
2.7.7.13 VCT1-3
-
Oryza sativa Japonica Group
2.7.7.13 VTC1-1
-
Oryza sativa Japonica Group
2.7.7.13 VTC1-3
-
Oryza sativa Japonica Group

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.13 37
-
assay at Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.13 7.5
-
assay at Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
2.7.7.13 Oryza sativa Japonica Group expression is inhibited by dark down
2.7.7.13 Oryza sativa Japonica Group the expression of OsVTC1-1 is inhibited in roots in the dark down
2.7.7.13 Oryza sativa Japonica Group the expression of OsVTC1-3 is inhibited in roots in the light down
2.7.7.13 Oryza sativa Japonica Group expression is induced by light up
2.7.7.13 Oryza sativa Japonica Group expression is induced by the dark up
2.7.7.13 Oryza sativa Japonica Group expression is induced by dark up
2.7.7.13 Oryza sativa Japonica Group the expression of OsVTC1-1 is induced in roots in the light up
2.7.7.13 Oryza sativa Japonica Group the expression of OsVTC1-3 is induced in roots in the dark up

General Information

EC Number General Information Comment Organism
2.7.7.13 malfunction overexpression of OsVTC1-8 does not restore the ascorbic acid synthesis of the vtc1-1 mutant in Arabidopsis Oryza sativa Japonica Group
2.7.7.13 metabolism enzyme OsVTC1-1 and OsVTC1-3 have higher GMPase activities than enzyme OsVTC1-8 in vitro. Only OsVTC1-1 and OsVTC1-3 are involved in ascorbic acid synthesis in rice Oryza sativa Japonica Group
2.7.7.13 physiological function in VTC1-1 RNAi plants, the ascorbic acid content of rice leaves decreases, and the ascorbic acid production induced by light is limited. The overexpression of isoform VTC1-1 or OsVTC1-3 restores the ascorbic acid synthesis of a vtc1-1 mutant in Arabidopsis thaliana Oryza sativa Japonica Group
2.7.7.13 physiological function VTC1-3 RNAi lines alter ascorbic acid synthesis levels in rice roots, but not in the leaves or under the light/dark treatment. The overexpression of isooform VTC1-1 or OsVTC1-3 restores the ascorbic acid synthesis of a vtc1-1 mutant in Arabidopsis thaliana Oryza sativa Japonica Group
2.7.7.13 physiological function enzyme OsVTC1-1 may be involved in the ascorbic acid synthesis, which takes place in leaves. Overexpression of OsVTC1-1 restores the ascorbic acid synthesis of the vtc1-1 mutant in Arabidopsis Oryza sativa Japonica Group
2.7.7.13 physiological function enzyme OsVTC1-3 may be responsible for ascorbic acid synthesis in roots. Overexpression of OsVTC1-3 restores the ascorbic acid synthesis of the vtc1-1 mutant in Arabidopsis Oryza sativa Japonica Group
2.7.7.13 physiological function the overexpression of VTC1-1 restores the ascorbic acid synthesis of a VTC1-1 mutant in Arabidopsis. In VTC1-1 RNAi plants, the ascorbic acid content of rice leaves decreases, and the ascorbic acid production induced by light is limited Oryza sativa Japonica Group
2.7.7.13 physiological function the overexpression of VTC1-3 restores the ascorbic acid synthesis of a VTC1-1 mutant in Arabidopsis. Isoform VTC1-3 RNAi lines alter ascorbic acid synthesis levels in rice roots, but not in the leaves or under the light/dark treatment Oryza sativa Japonica Group