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Literature summary extracted from

  • Laechler, K.; Imhof, J.; Reichelt, M.; Gershenzon, J.; Binder, S.
    The cytosolic branched-chain aminotransferases of Arabidopsis thaliana influence methionine supply, salvage and glucosinolate metabolism (2015), Plant Mol. Biol., 88, 119-131.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 1.08
-
3-methyl-2-oxopentanoate pH 8.3, 337°C Arabidopsis thaliana
2.6.1.42 2.64
-
4-methyl-2-oxopentanoate pH 8.3, 337°C Arabidopsis thaliana
2.6.1.42 2.69
-
3-methyl-2-oxobutanoate pH 8.3, 337°C Arabidopsis thaliana
2.6.1.42 2.83
-
4-methylthio-2-oxobutanoate pH 8.3, 337°C Arabidopsis thaliana
2.6.1.42 3.27
-
L-isoleucine pH 8.3, 337°C Arabidopsis thaliana
2.6.1.42 3.34
-
L-leucine pH 8.3, 337°C Arabidopsis thaliana
2.6.1.42 4.41
-
L-valine pH 8.3, 337°C Arabidopsis thaliana
2.6.1.42 6.58
-
L-methionine pH 8.3, 337°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.42 cytosol
-
Arabidopsis thaliana 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Arabidopsis thaliana Q9LPM9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 2-oxoglutarate + L-isoleucine
-
Arabidopsis thaliana L-glutamate + 3-methyl-2-oxopentanoate
-
r
2.6.1.42 2-oxoglutarate + L-leucine
-
Arabidopsis thaliana L-glutamate + 4-methyl-2-oxopentanoate
-
r
2.6.1.42 3-methyl-2-oxobutanoate + L-glutamate
-
Arabidopsis thaliana L-valine + 2-oxoglutarate
-
r
2.6.1.42 4-methylthio-2-oxobutanoate + L-glutamate
-
Arabidopsis thaliana L-methionine + 2-oxoglutarate
-
r
2.6.1.42 L-glutamate + 3-methyl-2-oxopentanoate
-
Arabidopsis thaliana 2-oxoglutarate + L-isoleucine
-
r
2.6.1.42 L-glutamate + 4-methyl-2-oxopentanoate
-
Arabidopsis thaliana 2-oxoglutarate + L-leucine
-
r
2.6.1.42 L-methionine + 2-oxoglutarate
-
Arabidopsis thaliana 4-methylthio-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate
-
Arabidopsis thaliana 3-methyl-2-oxobutanoate + L-glutamate
-
r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 At1g50110 locus name Arabidopsis thaliana
2.6.1.42 Bcat6
-
Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.6.1.42 physiological function mutant plants lacking the activities of isoforms bcat3, Bcat4, Bcat6 exhibit a clear macroscopic phenotype with smaller plants and abnormal leaf morphology. The triple mutant shows a dramatic reduction of Met-derived glucosinolate species down to 32 and 14% of wild-type levels in plant foliage and seeds, respectively, accompanied by a 46fold increase of free Met. 5?-Deoxy-5?-methylthioadenosine, an intermediate of the Met recycling pathway, accumulates to relative high amounts in the absence of the cytosolic Bcat4 and Bcat6 Arabidopsis thaliana