BRENDA - Enzyme Database

Extraplastidial cytidinediphosphate diacylglycerol synthase activity is required for vegetative development in Arabidopsis thaliana

Zhou, Y.; Peisker, H.; Weth, A.; Baumgartner, W.; Doermann, P.; Frentzen, M.; Plant J. 75, 867-879 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
Triton X-100
highest activity at 0.5%; highest activity at 0.5%; highest activity at 0.5%; stimulation, highest activity at 2-5 mM Mg2+ and 0.5% Triton X-100; stimulation, highest activity at 2–5 mM Mg2+ and 0.5% Triton X-100
Arabidopsis thaliana
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.7.41
transient expression of GFP-tagged CDS1 in tobacco leaf epidermal cells; transient expression of GFP-tagged CDS2 in tobacco leaf epidermal cells
Arabidopsis thaliana
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.7.41
additional information
generation of a T-DNA insertion (in the 5' untranslated region) mutant of CDS1, the cds1cds2 double mutant is seedling lethal, phenotypes, overview. The seedling-lethal phenotype of cds1cds2 double mutants can be rescued by inducing the expression of a functional CDS1 or CDS2 gene; generation of a T-DNA insertion (in the 5' untranslated region) mutant of CDS2, the cds1cds2 double mutant is seedling lethal, phenotypes, overview. The seedling-lethal phenotype of cds1cds2 double mutants can be rescued by inducing the expression of a functional CDS1 or CDS2 gene
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
endoplasmic reticulum
;
Arabidopsis thaliana
5783
-
2.7.7.41
endoplasmic reticulum membrane
membrane-bound; membrane-bound; membrane-bound
Arabidopsis thaliana
5789
-
2.7.7.41
microsome
-
Arabidopsis thaliana
-
-
2.7.7.41
additional information
the extraplastidial CDS proteins of Arabidopsis thaliana lack typical N-terminal targeting sequences; the extraplastidial CDS proteins of Arabidopsis thaliana lack typical N-terminal targeting sequences; the extraplastidial CDS proteins of Arabidopsis thaliana lack typical N-terminal targeting sequences
Arabidopsis thaliana
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
required, highest activity at 2-5 mM; required, highest activity at 2–5 mM; required, highest activity at 2–5 mM; required, highest activity at 2-5 mM Mg2+ and 0.5% Triton X-100; required, highest activity at 2–5 mM Mg2+ and 0.5% Triton X-100
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.41
CTP + phosphatidate
Arabidopsis thaliana
-
diphosphate + CDP-diacylglycerol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Arabidopsis thaliana
O04928
-
-
2.7.7.41
Arabidopsis thaliana
O49639
-
-
2.7.7.41
Arabidopsis thaliana
Q1PE48
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.41
additional information
constitutive expression; constitutive expression
Arabidopsis thaliana
-
2.7.7.41
seedling
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + 1,2-dioleoyl phosphatidic acid
-
739305
Arabidopsis thaliana
diphosphate + CDP-dioleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-dipalmitoyl phosphatidic acid
best substrate
739305
Arabidopsis thaliana
diphosphate + CDP-dipalmitoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-palmitoyl-2-oleoyl phosphatidic acid
best substrate
739305
Arabidopsis thaliana
diphosphate + CDP-1-palmitoyl-2-oleoylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
-
739305
Arabidopsis thaliana
diphosphate + CDP-diacylglycerol
-
-
-
?
2.7.7.41
additional information
decreasing activity in the order 1,2-dioleoyl phosphatidic acid, 1-palmitoyl-2-oleoyl phosphatidic acid, 1,2-dipalmitoyl phosphatidic acid
739305
Arabidopsis thaliana
?
-
-
-
-
2.7.7.41
additional information
the extraplastidial isoforms are most active with the 18:1/18:1 species of phosphatidates and their activities decreases in the order of 16:0/18:1 phosphatidate to 16:0/16:0 phosphatidate
739305
Arabidopsis thaliana
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
7.5
-
-
Arabidopsis thaliana
2.7.7.41
8
-
;
Arabidopsis thaliana
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
Triton X-100
highest activity at 0.5%; stimulation, highest activity at 2-5 mM Mg2+ and 0.5% Triton X-100
Arabidopsis thaliana
2.7.7.41
Triton X-100
highest activity at 0.5%; stimulation, highest activity at 2–5 mM Mg2+ and 0.5% Triton X-100
Arabidopsis thaliana
2.7.7.41
Triton X-100
highest activity at 0.5%
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.41
transient expression of GFP-tagged CDS1 in tobacco leaf epidermal cells
Arabidopsis thaliana
2.7.7.41
transient expression of GFP-tagged CDS2 in tobacco leaf epidermal cells
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.7.41
additional information
generation of a T-DNA insertion (in the 5' untranslated region) mutant of CDS2, the cds1cds2 double mutant is seedling lethal, phenotypes, overview. The seedling-lethal phenotype of cds1cds2 double mutants can be rescued by inducing the expression of a functional CDS1 or CDS2 gene
Arabidopsis thaliana
2.7.7.41
additional information
generation of a T-DNA insertion (in the 5' untranslated region) mutant of CDS1, the cds1cds2 double mutant is seedling lethal, phenotypes, overview. The seedling-lethal phenotype of cds1cds2 double mutants can be rescued by inducing the expression of a functional CDS1 or CDS2 gene
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
endoplasmic reticulum
-
Arabidopsis thaliana
5783
-
2.7.7.41
endoplasmic reticulum membrane
membrane-bound
Arabidopsis thaliana
5789
-
2.7.7.41
microsome
-
Arabidopsis thaliana
-
-
2.7.7.41
additional information
the extraplastidial CDS proteins of Arabidopsis thaliana lack typical N-terminal targeting sequences
Arabidopsis thaliana
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
required, highest activity at 2–5 mM; required, highest activity at 2-5 mM Mg2+ and 0.5% Triton X-100
Arabidopsis thaliana
2.7.7.41
Mg2+
required, highest activity at 2-5 mM; required, highest activity at 2–5 mM Mg2+ and 0.5% Triton X-100
Arabidopsis thaliana
2.7.7.41
Mg2+
required, highest activity at 2–5 mM
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.41
CTP + phosphatidate
Arabidopsis thaliana
-
diphosphate + CDP-diacylglycerol
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.41
additional information
constitutive expression
Arabidopsis thaliana
-
2.7.7.41
seedling
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + 1,2-dioleoyl phosphatidic acid
-
739305
Arabidopsis thaliana
diphosphate + CDP-dioleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-dipalmitoyl phosphatidic acid
best substrate
739305
Arabidopsis thaliana
diphosphate + CDP-dipalmitoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-palmitoyl-2-oleoyl phosphatidic acid
best substrate
739305
Arabidopsis thaliana
diphosphate + CDP-1-palmitoyl-2-oleoylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
-
739305
Arabidopsis thaliana
diphosphate + CDP-diacylglycerol
-
-
-
?
2.7.7.41
additional information
decreasing activity in the order 1,2-dioleoyl phosphatidic acid, 1-palmitoyl-2-oleoyl phosphatidic acid, 1,2-dipalmitoyl phosphatidic acid
739305
Arabidopsis thaliana
?
-
-
-
-
2.7.7.41
additional information
the extraplastidial isoforms are most active with the 18:1/18:1 species of phosphatidates and their activities decreases in the order of 16:0/18:1 phosphatidate to 16:0/16:0 phosphatidate
739305
Arabidopsis thaliana
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
7.5
-
-
Arabidopsis thaliana
2.7.7.41
8
-
-
Arabidopsis thaliana
General Information
EC Number
General Information
Commentary
Organism
2.7.7.41
evolution
isoforms of CDS are located in plastids, mitochondria and the endomembrane system of plants and are encoded by five genes in Arabidopsis. Two genes have previously been shown to code for the plastidial isoforms which are indispensable for the biosynthesis of plastidial phosphatidylglycerol, and thus biogenesis and function of thylakoid membranes. the extraplastidial CDS isoforms, encoded by CDS1 and CDS2 which are constitutively expressed contrary to CDS3. These closely related CDS genes code for membrane proteins located in the endoplasmic reticulum and possess very similar enzymatic properties; isoforms of CDS are located in plastids, mitochondria and the endomembrane system of plants and are encoded by five genes in Arabidopsis. Two genes have previously been shown to code for the plastidial isoforms which are indispensable for the biosynthesis of plastidial phosphatidylglycerol, and thus biogenesis and function of thylakoid membranes. the extraplastidial CDS isoforms, encoded by CDS1 and CDS2 which are constitutively expressed contrary to CDS3. These closely related CDS genes code for membrane proteins located in the endoplasmic reticulum and possess very similar enzymatic properties; isoforms of CDS are located in plastids, mitochondria and the endomembrane system of plants and are encoded by five genes in Arabidopsis. Two genes have previously been shown to code for the plastidial isoforms which are indispensable for the biosynthesis of plastidial phosphatidylglycerol, and thus biogenesis and function of thylakoid membranes. the extraplastidial CDS isoforms, encoded by CDS1 and CDS2 which are constitutively expressed contrary to CDS3. These closely related CDS genes code for membrane proteins located in the endoplasmic reticulum and possess very similar enzymatic properties
Arabidopsis thaliana
2.7.7.41
malfunction
the seedling lethal phenotype of the cds1cds2 double mutant shows that plant cells require at least one catalytically active microsomal CDS isoform for cell division and expansion. According to the altered glycerolipid composition of the double mutant in comparison with wild-type seedlings, it is likely that the drastic decrease in the level of phosphatidylinositol and the increase in phosphatidic acid cause defects in cell division and expansion. The cds1cds2 double mutant is seedling lethal, phenotypes, overview. The seedling-lethal phenotype of cds1cds2 double mutants can be rescued by inducing the expression of a functional CDS1 or CDS2 gene; the seedling lethal phenotype of the cds1cds2 double mutant shows that plant cells require at least one catalytically active microsomal CDS isoform for cell division and expansion. According to the altered glycerolipid composition of the double mutant in comparison with wild-type seedlings, it is likely that the drastic decrease in the level of phosphatidylinositol and the increase in phosphatidic acid cause defects in cell division and expansion. The cds1cds2 double mutant is seedling lethal, phenotypes, overview. The seedling-lethal phenotype of cds1cds2 double mutants can be rescued by inducing the expression of a functional CDS1 or CDS2 gene
Arabidopsis thaliana
2.7.7.41
physiological function
Arabidopsis mutants lacking either one or both CDS1 and CDS2 genes clearly show that these two genes have redundant functions. As reflected in the seedling lethal phenotype of the cds1cds2 double mutant, plant cells require at least one catalytically active microsomal CDS isoform for cell division and expansion; Arabidopsis mutants lacking either one or both CDS1 and CDS2 genes clearly show that these two genes have redundant functions. As reflected in the seedling lethal phenotype of the cds1cds2 double mutant, plant cells require at least one catalytically active microsomal CDS isoform for cell division and expansion; cytidine diphosphate diacylglycerol synthase (CDS) catalyzes the activation of phosphatidic acid to CDP-diacylglycerol, a central intermediate in glycerolipid biosynthesis in prokaryotic and eukaryotic organisms. CDP-diacylglycerol is the precursor to phosphatidylinositol, phosphatidylglycerol, and cardiolipin of eukaryotic phospholipids that are essential for various cellular functions; cytidine diphosphate diacylglycerol synthase (CDS) catalyzes the activation of phosphatidic acid to CDP-diacylglycerol, a central intermediate in glycerolipid biosynthesis in prokaryotic and eukaryotic organisms. CDP-diacylglycerol is the precursor to phosphatidylinositol, phosphatidylglycerol, and cardiolipin of eukaryotic phospholipids that are essential for various cellular functions; cytidine diphosphate diacylglycerol synthase (CDS) catalyzes the activation of phosphatidic acid to CDP-diacylglycerol, a central intermediate in glycerolipid biosynthesis in prokaryotic and eukaryotic organisms. CDP-diacylglycerol is the precursor to phosphatidylinositol, phosphatidylglycerol, and cardiolipin of eukaryotic phospholipids that are essential for various cellular functions
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.7.41
evolution
isoforms of CDS are located in plastids, mitochondria and the endomembrane system of plants and are encoded by five genes in Arabidopsis. Two genes have previously been shown to code for the plastidial isoforms which are indispensable for the biosynthesis of plastidial phosphatidylglycerol, and thus biogenesis and function of thylakoid membranes. the extraplastidial CDS isoforms, encoded by CDS1 and CDS2 which are constitutively expressed contrary to CDS3. These closely related CDS genes code for membrane proteins located in the endoplasmic reticulum and possess very similar enzymatic properties
Arabidopsis thaliana
2.7.7.41
malfunction
the seedling lethal phenotype of the cds1cds2 double mutant shows that plant cells require at least one catalytically active microsomal CDS isoform for cell division and expansion. According to the altered glycerolipid composition of the double mutant in comparison with wild-type seedlings, it is likely that the drastic decrease in the level of phosphatidylinositol and the increase in phosphatidic acid cause defects in cell division and expansion. The cds1cds2 double mutant is seedling lethal, phenotypes, overview. The seedling-lethal phenotype of cds1cds2 double mutants can be rescued by inducing the expression of a functional CDS1 or CDS2 gene
Arabidopsis thaliana
2.7.7.41
physiological function
Arabidopsis mutants lacking either one or both CDS1 and CDS2 genes clearly show that these two genes have redundant functions. As reflected in the seedling lethal phenotype of the cds1cds2 double mutant, plant cells require at least one catalytically active microsomal CDS isoform for cell division and expansion; cytidine diphosphate diacylglycerol synthase (CDS) catalyzes the activation of phosphatidic acid to CDP-diacylglycerol, a central intermediate in glycerolipid biosynthesis in prokaryotic and eukaryotic organisms. CDP-diacylglycerol is the precursor to phosphatidylinositol, phosphatidylglycerol, and cardiolipin of eukaryotic phospholipids that are essential for various cellular functions
Arabidopsis thaliana
2.7.7.41
physiological function
cytidine diphosphate diacylglycerol synthase (CDS) catalyzes the activation of phosphatidic acid to CDP-diacylglycerol, a central intermediate in glycerolipid biosynthesis in prokaryotic and eukaryotic organisms. CDP-diacylglycerol is the precursor to phosphatidylinositol, phosphatidylglycerol, and cardiolipin of eukaryotic phospholipids that are essential for various cellular functions
Arabidopsis thaliana