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Literature summary extracted from

  • Vom Dorp, K.; Hoelzl, G.; Plohmann, C.; Eisenhut, M.; Abraham, M.; Weber, A.P.; Hanson, A.D.; Doermann, P.
    Remobilization of phytol from chlorophyll degradation is essential for tocopherol synthesis and growth of Arabidopsis (2015), Plant Cell, 27, 2846-2859.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.182 gene VTE5, recombinant functional expression of gene VTE5 or functional coexpression of genes VTE5 and VTE6 in Escherichia coli, quantitative real-time PCR expression analysis Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.182 additional information construction of an independent mutant allele (pst12490, vte5-2) that carries a transposon insertion in the first exon behind the start codon of the VTE5 gene and shows no vte5 expression and absence of VTE5 mRNA. Construction of VTE5/VTE6 double mutant plants, which are tocopherol-deficient and contain more chlorophyll, but reduced amounts of phytol and phytyl-phosphate compared with vte6 mutants, suggesting that phytol or phytyl-phosphate are detrimental to plant growth. The growth defect of vte6-1 is alleviated in the vte5-2/vte6-1 double mutant. The tocopherol-deficient mutants vte1, vte2, vte4, and vte5 can grow on soil and produce fertile seeds, while vte6-1 and vte6-2 cannot grow photoautotrophically and are infertile. Amounts of phytol are unchanged in vte5-2 and vte5-2/vte6-1 mutants compared with the wild-type, the different forms of fatty acid phytyl esters are increased in leaves of vte5-2, vte6-1, vte6-2, and vte5-2 vte6-1 compared with the wild type, especially 12:0-phytol, 14:0-phytol, and 16:0-phytol Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.182 chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.182 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.182 CTP + phytol Arabidopsis thaliana
-
 CDP + phytyl phosphate phytyl phosphate is phosphorylated by phytyl phosphate kinase, VTE6 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.182 Arabidopsis thaliana Q9LZ76 gene VTE5 or At5g04490
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.182 leaf
-
 Arabidopsis thaliana
-
2.7.1.182 seed
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.182 CTP + phytol
-
 Arabidopsis thaliana CDP + phytyl phosphate phytyl phosphate is phosphorylated by phytyl phosphate kinase, VTE6 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.182 VTE5
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.182 30
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.182 CTP
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.7.1.182 malfunction the growth defect of vte6-1 is alleviated in the vte5-2/vte6-1 double mutant. The tocopherol-deficient mutants vte1, vte2, vte4, and vte5 can grow on soil and produce fertile seeds, while vte6-1 and vte6-2 cannot grow photoautotrophically and are infertile. Amounts of phytol are unchanged in vte5-2 and vte5-2/vte6-1 mutants compared with the wild-type Arabidopsis thaliana
2.7.1.182 metabolism phytol from chlorophyll degradation can be phosphorylated to phytyl-phosphate by phytol kinase, EC 2.7.1.182, and to phytyl-diphosphate, the substrate for tocopherol (vitamin E) synthesis by phytyl-diphosphate kinase, EC 2.7.4.BX. Tocopherol synthesis in leaves depends on phytol derived from chlorophyll, not on de novo synthesis of phytyl-diphosphate from geranylgeranyl-diphosphate. The phytol phosphorylation pathway is essential for plant growth and development Arabidopsis thaliana
2.7.1.182 physiological function the essential enzyme is required for tocopherol synthesis and photoautotrophic growth synthesizing phytyl phosphate from phytol from degenerated chlorophyll in the phytol phosphorylation pathway Arabidopsis thaliana