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Literature summary extracted from

  • Lin, Y.C.; Liu, Y.C.; Nakamura, Y.
    The choline/ethanolamine kinase family in Arabidopsis: essential role of CEK4 in phospholipid biosynthesis and embryo development (2015), Plant Cell, 27, 1497-1511.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.82 gene At2g26830, PCR-based genotyping of CEK family members CEK1-4 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.82 additional information construction of CEK4 knockout mutant, the CEK4 knockout causes embryonic lethality, and developmental arrest after the heart stage, with no defect in the pollen Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.82 additional information the enzyme has no transmembrane domain Arabidopsis thaliana
-
-
2.7.1.82 plasma membrane mainly Arabidopsis thaliana 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.82 40000
-
 x * 40000, about, sequence calculation Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.82 ATP + ethanolamine Arabidopsis thaliana
-
 ADP + O-phosphoethanolamine
-
 ?
2.7.1.82 additional information Arabidopsis thaliana enzyme CEK4 is a bifunctional choline/ethanolamine kinase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.82 Arabidopsis thaliana O81024 gene At2g26830
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.82 embryo
-
 Arabidopsis thaliana
-
2.7.1.82 additional information enzyme CEK4 is preferentially expressed in the vasculature, organ boundaries, and mature embryos. Isozyme CEK4 shows increased expression in senescent tissues Arabidopsis thaliana
-
2.7.1.82 pollen high expression level of isozyme CEK4 in male reproductive organs Arabidopsis thaliana
-
2.7.1.82 seedling
-
 Arabidopsis thaliana
-
2.7.1.82 silique
-
 Arabidopsis thaliana
-
2.7.1.82 vascular system
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.82 ATP + ethanolamine
-
 Arabidopsis thaliana ADP + O-phosphoethanolamine
-
 ?
2.7.1.82 additional information enzyme CEK4 is a bifunctional choline/ethanolamine kinase Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.82 ? x * 40000, about, sequence calculation Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.1.82 CEK4
-
 Arabidopsis thaliana
2.7.1.82 choline/ethanolamine kinase
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.82 ATP
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.7.1.82 evolution the enzmye belongs to the choline/ethanolamine kinase (CEK) family catalyzing the initial steps of phospholipid biosynthesis Arabidopsis thaliana
2.7.1.82 malfunction knocking out of CEK4 caused an embryo-lethal phenotype with defective embryo development, which is complemented by transformation of genomic CEK4. Overexpression of enzyme CEK4 in wild-type Arabidopsis thaliana increases the levels of phosphatidylcholine in seedlings and mature siliques and of major membrane lipids in seedlings and triacylglycerol in mature siliques Arabidopsis thaliana
2.7.1.82 metabolism the enzyme catalyzes the initial steps of phospholipid biosynthesis Arabidopsis thaliana
2.7.1.82 physiological function enzyme CEK4 may be the plasma membrane-localized isoform of the CEK family involved in the rate-limiting step of phosphatidylcholine biosynthesis and appears to be required for embryo development in Arabidopsis thaliana Arabidopsis thaliana