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Literature summary extracted from

  • Seferoglu, A.B.; Koper, K.; Can, F.B.; Cevahir, G.; Kavakli, I.H.
    Enhanced heterotetrameric assembly of potato ADP-glucose pyrophosphorylase using reverse genetics (2014), Plant Cell Physiol., 55, 1473-1483.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate A0.5 value for wild-type 0.28 mm, for large subunit E370G/small subunit wild-type 0.83 mM Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 E370G mutation in the large subunit alters the heterotetrameric stability along with the binding properties of substrate and effectors of the enzyme. The affinity of the large subunit E370G/small subunit wild-type AGPase for glucose-1-phosphate is 3fold less than for wild type AGPase. The mutant enzyme complex requires 3fold more 3-phosphogyceric acid to be activated and lis less heat stable Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 phosphate
-
 Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.044
-
 alpha-D-glucose 1-phosphate wild-type, pH 7.4, 37°C Solanum tuberosum
2.7.7.27 0.05
-
 ATP wild-type, pH 7.4, 37°C Solanum tuberosum
2.7.7.27 0.09
-
 ATP large subunit E370G/small subunit wild-type, pH 7.4, 37°C Solanum tuberosum
2.7.7.27 0.13
-
 alpha-D-glucose 1-phosphate large subunit E370G/small subunit wild-type, pH 7.4, 37°C Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Solanum tuberosum Q00081 large subbunit
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.27 0.15
-
 large subunit E370G/small subunit wild-type, presence of 1 mM 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum phosphate
2.7.7.27 0.38
-
 large subunit E370G/small subunit wild-type, presence of 2.5 mM 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum phosphate
2.7.7.27 0.44
-
 wild-type, presence of 1 mM 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum phosphate
2.7.7.27 0.67
-
 wild-type, presence of 2.5 mM 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum phosphate