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Literature summary extracted from

  • Chen, J.; Gao, L.; Baek, D.; Liu, C.; Ruan, Y.; Shi, H.
    Detoxification function of the Arabidopsis sulphotransferase AtSOT12 by sulphonation of xenobiotics (2015), Plant Cell Environ., 38, 1673-1682.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.2 expressed in Saccharomyces cerevisiae strain w303-1a Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.2 0.01
-
 cycloheximide at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.035
-
 o-Cresol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.135
-
 4-Ethylphenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.152
-
 3-Ethylphenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.18
-
 m-cresol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.276
-
 4-nitrophenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.312
-
 2-Nitrophenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.475
-
 3-Nitrophenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.494
-
 p-Cresol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 1.16
-
 2-Ethylphenol at pH 7.5 and 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.2 cytosol
-
 Arabidopsis thaliana 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.2 Arabidopsis thaliana P52839
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.2 root
-
 Arabidopsis thaliana
-
2.8.2.2 seedling
-
 Arabidopsis thaliana
-
2.8.2.2 shoot
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.2 3'-phosphoadenylylsulfate + 1,10-phenanthroline
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 1-hydroxypyrene
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 1-hydroxypyrene sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 1-naphthol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 1-pyrenemethanol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 1-pyrenemethylsulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 2-aminofluorene
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 2-aminofluorene sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 2-aminophenol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 2-aminophenyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 2-ethylphenol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 2-ethylphenyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 2-hydroxyestradiol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 2-hydroxyestradiol sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 2-naphthol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 2-nitrophenol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 2-nitrophenyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 3-aminophenol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 3-aminophenyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 3-ethylphenol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 3-ethylphenyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 3-nitrophenol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 3-nitrophenyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 4-aminophenol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 4-aminophenyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 4-ethylphenol best substrate Arabidopsis thaliana adenosine 3',5'-bisphosphate + 4-ethylphenyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + 5-sulfosalicylic acid
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 5-sulfo-2-(sulfooxy)benzoic acid
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + beta-estradiol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + beta-estradiol 3-O-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + cycloheximide
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + cycloheximide sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + hycanthone
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + hycanthone sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + hydroquinone
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + hydroquinone sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + m-cresol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + m-cresol sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + o-cresol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + o-cresol sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + p-cresol
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + p-cresol sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + sodium 4-amino-salicylate
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + sodium 4-amino-salicylate sulfate
-
 ?
2.8.2.2 additional information no activity with pentachlorophenol Arabidopsis thaliana ?
-
 ?
2.8.2.2 additional information the enzyme displays relatively low activities to steroid compounds, and although the enzyme sulfonates a variety of phenolic compounds, it has selectivity towards side chains and their locations at the phenolic ring Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.2 SOT12
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.2.2 0.000035
-
 o-Cresol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.00004
-
 m-cresol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000053
-
 3-Ethylphenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000109
-
 4-Ethylphenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000112
-
 2-Ethylphenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000128
-
 p-Cresol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000389
-
 3-Nitrophenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000459
-
 2-Nitrophenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000545
-
 4-nitrophenol at pH 7.5 and 30°C Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.8.2.2 physiological function isoform SOT12 is required for detoxification of cycloheximide Arabidopsis thaliana

KCat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8.2.2 0.000097
-
 2-Ethylphenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000222
-
 m-cresol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000259
-
 p-Cresol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000347
-
 3-Ethylphenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000812
-
 4-Ethylphenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000819
-
 3-Nitrophenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.000849
-
 o-Cresol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.00147
-
 2-Nitrophenol at pH 7.5 and 30°C Arabidopsis thaliana
2.8.2.2 0.001975
-
 4-nitrophenol at pH 7.5 and 30°C Arabidopsis thaliana