EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.11 | gene ZmPIS, cloning of the gene ZmPIS in sense or antisense orientation in the T-DNA region of the vector, driven by a maize-constitutive ubiquitin promoter, overexpression of the enzyme in transgenic Zea mays, and quantitative RT-PCR expression analysis | Zea mays |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.8.11 | additional information | generation of transgenic maize constitutively overexpressing or underexpressing gene ZmPIS, encoding PIS from maize, under the control of a maize ubiquitin promoter. Lipid profiling analysis shows that, under drought stress conditions, the overexpression of ZmPIS in maize results in significantly elevated levels of most phospholipids and galactolipids in leaves compared with those in wild-type plants, analysis of expression pattern of genes involved in the phospholipid metabolism pathway and drought response, phenotype, overview. Sense transgenic plants are more tolerant to drought stress at the pre-flowering stage and retain more solutes and water under drought stress conditions | Zea mays |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.8.11 | membrane | - |
Zea mays | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.11 | CDP-diacylglycerol + myo-inositol | Zea mays | - |
CMP + 1-phosphatidyl-1D-myo-inositol | - |
? | |
2.7.8.11 | CDP-diacylglycerol + myo-inositol | Zea mays DH4866 | - |
CMP + 1-phosphatidyl-1D-myo-inositol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.11 | Zea mays | Q6UG58 | - |
- |
2.7.8.11 | Zea mays DH4866 | Q6UG58 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.8.11 | leaf | - |
Zea mays | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Zea mays | CMP + 1-phosphatidyl-1D-myo-inositol | - |
? | |
2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Zea mays DH4866 | CMP + 1-phosphatidyl-1D-myo-inositol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.8.11 | phosphatidylinositol synthase | - |
Zea mays |
2.7.8.11 | PtdIns synthase | - |
Zea mays |
2.7.8.11 | ZmPIS | - |
Zea mays |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.8.11 | malfunction | overexpression of the phosphatidylinositol synthase gene (ZmPIS) confers drought stress tolerance by altering membrane lipid composition and increasing abscisic acid synthesis in maize. Lipid profiling analysis shows that, under drought stress conditions, the overexpression of ZmPIS in maize results in significantly elevated levels of most phospholipids and galactolipids in leaves compared with those in wild-type. The expression of some genes involved in the phospholipid metabolism pathway and the abscisic acid biosynthesis pathway including ZmPLC, ZmPLD, ZmDGK1, ZmDGK3, ZmPIP5K9, ZmABA1, ZmNCED, ZmAAO1, ZmAAO2 and ZmSCA1, is markedly upregulated in the overexpression lines after drought stress. The drought stress tolerance of the ZmPIS sense transgenic plants is enhanced significantly at the preflowering stages compared with wild-type maize plants | Zea mays |
2.7.8.11 | metabolism | phosphatidylinositol synthase is a key enzyme in the phospholipid pathway, it catalyzes the first step of the phosphatidylinositol signalling pathway | Zea mays |
2.7.8.11 | physiological function | the enzyme is responsible for phosphatidylinositol synthesis, which is not only a structural component of cell membranes, but also the precursor of the phospholipid signal molecules that regulate plant response to environment stresses. ZmPIS regulates the plant response to drought stress through altering membrane lipid composition and increasing abscisic acid synthesis in maize | Zea mays |