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Literature summary extracted from

  • Sievert, C.; Beuerle, T.; Hollmann, J.; Ober, D.
    Single cell subtractive transcriptomics for identification of cell-specifically expressed candidate genes of pyrrolizidine alkaloid biosynthesis (2015), Phytochemistry, 117, 17-24.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.45 gene hss1, DNA and amino acid sequence determination and analysis, cloning from cDNA library of lower leaf epidermis cells from leaves and stems, quantitative real-time PCR expression analysis in epidermis cells Heliotropium indicum
2.5.1.45 gene hss1, DNA and amino acid sequence determination and analysis, cloning from cDNA library of lower leaf epidermis cells from leaves and stems, quantitative real-time PCR expression analysis in epidermis cells Cynoglossum officinale
2.5.1.45 gene hss1, DNA and amino acid sequence determination and analysis, cloning from cDNA library of lower leaf epidermis cells from leaves and stems, quantitative real-time PCR expression analysis in epidermis cells Symphytum officinale

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.45 spermidine + putrescine Heliotropium indicum
-
 sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 spermidine + putrescine Cynoglossum officinale
-
 sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 spermidine + putrescine Symphytum officinale
-
 sym-homospermidine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.45 Cynoglossum officinale Q5ZN66 gene hss1
-
2.5.1.45 Heliotropium indicum Q5ZN63 gene hss1
-
2.5.1.45 Symphytum officinale Q5ZN55 gene hss1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.45 leaf the enzyme is expressed only in the cells of the lower leaf epidermis and the epidermis of the stem Heliotropium indicum
-
2.5.1.45 leaf the enzyme is expressed only in the cells of the lower leaf epidermis and the epidermis of the stem Cynoglossum officinale
-
2.5.1.45 leaf the enzyme is expressed only in the cells of the lower leaf epidermis and the epidermis of the stem Symphytum officinale
-
2.5.1.45 plant epidermis from lower epidermis of leaf and stem, no activity in upper epidermis cells Heliotropium indicum
-
2.5.1.45 plant epidermis from lower epidermis of leaf and stem, no activity in upper epidermis cells Cynoglossum officinale
-
2.5.1.45 plant epidermis from lower epidermis of leaf and stem, no activity in upper epidermis cells Symphytum officinale
-
2.5.1.45 stem the enzyme is expressed only in the cells of the lower leaf epidermis and the epidermis of the stem Heliotropium indicum
-
2.5.1.45 stem the enzyme is expressed only in the cells of the lower leaf epidermis and the epidermis of the stem Cynoglossum officinale
-
2.5.1.45 stem the enzyme is expressed only in the cells of the lower leaf epidermis and the epidermis of the stem Symphytum officinale
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.45 spermidine + putrescine
-
 Heliotropium indicum sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 spermidine + putrescine
-
 Cynoglossum officinale sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 spermidine + putrescine
-
 Symphytum officinale sym-homospermidine + propane-1,3-diamine
-
 ?

Expression

EC Number Organism Comment Expression
2.5.1.45 Heliotropium indicum no effect on enzyme expression by methyl jasmonate additional information
2.5.1.45 Cynoglossum officinale no effect on enzyme expression by methyl jasmonate additional information
2.5.1.45 Symphytum officinale no effect on enzyme expression by methyl jasmonate additional information

General Information

EC Number General Information Comment Organism
2.5.1.45 metabolism the enzyme is first specific enzyme in the biosynthesis of pyrrolizidine alkaloids Cynoglossum officinale
2.5.1.45 metabolism the enzyme is first specific enzyme in the biosynthesis of pyrrolizidine alkaloids Symphytum officinale
2.5.1.45 metabolism the enzyme is first specific enzyme in the biosynthesis of pyrrolizidine alkaloids, it is involved in the biosynthetic pathway of the lycopsamine-type polyamine indicine N-oxide Heliotropium indicum