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Literature summary extracted from

  • Takeda, T.; Nakano, Y.; Takahashi, M.; Konno, N.; Sakamoto, Y.; Arashida, R.; Marukawa, Y.; Yoshida, E.; Ishikawa, T.; Suzuki, K.
    Identification and enzymatic characterization of an endo-1,3-beta-glucanase from Euglena gracilis (2015), Phytochemistry, 116, 21-27.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expression in Aspergillus oryzae Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.39 Co2+ 1 mM, 40-50% inhibition Euglena gracilis
3.2.1.39 Cu2+ 1 mM, 40-50% inhibition Euglena gracilis
3.2.1.39 Fe2+ 1 mM, 40-50% inhibition Euglena gracilis
3.2.1.39 Mn2+ 1 mM, 40-50% inhibition Euglena gracilis
3.2.1.39 additional information not inhibitory: NaCl up to 100 mM, or 1 mM MgCl2, KCl, CaCl2 and ZnSO4 Euglena gracilis
3.2.1.39 Ni2+ 1 mM, 85% inhibition Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 Laminarin Km value 0.21 mg/ml, pH 5.0, 50°C Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 40000
-
-
 Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Euglena gracilis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.39 proteolytic modification sequence contains a putative signal peptide consisting of 16 amino acids Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 alkaline-treated paramylon + H2O 78.6% of the activity with laminarin Euglena gracilis ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Euglena gracilis D-glucose + laminaribiose + laminaritriose
-
 ?
3.2.1.39 additional information enzyme additionally catalyzes a transglycosylation reaction by which reaction products with a higher molecular weight than the supplied substrates are initially generated. Ultimately the substrates are degraded into glucose, laminaribiose and laminaritriose Euglena gracilis ?
-
 ?
3.2.1.39 paramylon + H2O 23.4% of the activity with laminarin Euglena gracilis ? the enzyme effectively produces soluble beta-1,3-glucans from alkaline-treated Euglena freeze-dried powder containing paramylon ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 40000, SDS-PAGE and calculated Euglena gracilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 Cel17A
-
 Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 60
-
-
 Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 4 5.5
-
 Euglena gracilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.39 Euglena gracilis calculated
-
 4.3