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Literature summary extracted from

  • Pavlidi, N.; Tseliou, V.; Riga, M.; Nauen, R.; Van Leeuwen, T.; Labrou, N.E.; Vontas, J.
    Functional characterization of glutathione S-transferases associated with insecticide resistance in Tetranychus urticae (2015), Pestic. Biochem. Physiol., 121, 53-60.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli BL21(DE3) cells Tetranychus urticae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 abamectin competitive inhibition (38.46% at 0.05 mM) of isoform GSTd14 Tetranychus urticae
2.5.1.18 bifenthrin 7.27% inhibition of isoform GSTd10 at 0.05 mM Tetranychus urticae
2.5.1.18 clofentezine 28.05% inhibition of isoform GSTd14 at 0.05 mM and 27.68% inhibition of isoform GSTm09 at 0.05 mM Tetranychus urticae
2.5.1.18 hexythiazox 11.21% inhibition of isoform GSTd14 at 0.05 mM and 12.94% inhibition of isoform GSTm09 at 0.05 mM Tetranychus urticae
2.5.1.18 pyridaben 25.84% inhibition of isoform GSTm09 at 0.05 mM Tetranychus urticae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.26
-
 1-chloro-2,4-dinitrobenzene isoform GSTm09, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.33
-
 glutathione isoform GSTd10, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.84
-
 1-chloro-2,4-dinitrobenzene isoform GSTd10, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 1.69
-
 1-chloro-2,4-dinitrobenzene isoform GSTd14, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 2.34
-
 glutathione isoform GSTm09, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 3.79
-
 glutathione isoform GSTd14, at pH 6.5 and 25°C Tetranychus urticae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Tetranychus urticae
-
 two-spotted spider mite
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 Ni-NTA column chromatography Tetranychus urticae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.01
-
 isoform GSTd10, with 1,2-dicloro-4-nitrobenzene as substrate, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.08
-
 isoform GSTd14, with 1,2-dicloro-4-nitrobenzene as substrate, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.09
-
 isoform GSTm09, with 1,2-dicloro-4-nitrobenzene as substrate, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.1
-
 isoform GSTd14, with cumene hydroperoxide as substrate, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.34
-
 isoform GSTd10, with cumene hydroperoxide as substrate, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.69
-
 isoform GSTd14, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 1.1
-
 isoform GSTd10, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 3.34
-
 isoform GSTm09, with cumene hydroperoxide as substrate, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 15.94
-
 isoform GSTm09, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C Tetranychus urticae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1,2-dichloro-4-nitrobenzene + glutathione low activity Tetranychus urticae ?
-
 ?
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione best substrate Tetranychus urticae S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 cumene hydroperoxide + glutathione
-
 Tetranychus urticae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Tetranychus urticae
2.5.1.18 GSTD10 isoform Tetranychus urticae
2.5.1.18 GSTd14 isoform Tetranychus urticae
2.5.1.18 GSTm09 isoform Tetranychus urticae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.02
-
 glutathione isoform GSTd14, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.027
-
 glutathione isoform GSTd10, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.03
-
 1-chloro-2,4-dinitrobenzene isoform GSTd14, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.051
-
 1-chloro-2,4-dinitrobenzene isoform GSTd10, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.25
-
 1-chloro-2,4-dinitrobenzene isoform GSTm09, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.39
-
 glutathione isoform GSTm09, at pH 6.5 and 25°C Tetranychus urticae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.005
-
 glutathione isoform GSTd14, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.018
-
 1-chloro-2,4-dinitrobenzene isoform GSTd14, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.06
-
 1-chloro-2,4-dinitrobenzene isoform GSTd10, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.08
-
 glutathione isoform GSTd10, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.17
-
 glutathione isoform GSTm09, at pH 6.5 and 25°C Tetranychus urticae
2.5.1.18 0.94
-
 1-chloro-2,4-dinitrobenzene isoform GSTm09, at pH 6.5 and 25°C Tetranychus urticae