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Literature summary extracted from

  • Matt, J.; Duchene, M.
    Molecular and biochemical characterization of Entamoeba histolytica fructokinase (2015), Parasitol. Res., 114, 1939-1947.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.4 expression in Escherichia coli Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.4 D-fructose substrate inhibition, inhibitory above 1 mM Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.156
-
 D-fructose pH 7, 37°C Entamoeba histolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.4 Mg2+ required, optimum concentration 10 mM Entamoeba histolytica
2.7.1.4 Mn2+ 83% of the activity with Mg2+ Entamoeba histolytica
2.7.1.4 additional information no activity with Ca2+ Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.4 33600
-
-
 Entamoeba histolytica
2.7.1.4 35000
-
-
 Entamoeba histolytica

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Entamoeba histolytica C4M2I2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.4 26.3
-
 pH 7, 37°C Entamoeba histolytica

Storage Stability

EC Number Storage Stability Organism
2.7.1.4 -20°C in 50% (v/v) glycerol, stable for at least 6 months Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose
-
 Entamoeba histolytica ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ATP + D-mannose
-
 Entamoeba histolytica ADP + D-mannose 6-phosphate
-
 ?
2.7.1.4 additional information no substrates: glucose, galactose Entamoeba histolytica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.4 ? x * 35000, SDS-PAGE, x * 33600, calculated Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
2.7.1.4 CL6EHI_054510
-
 Entamoeba histolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.4 37
-
-
 Entamoeba histolytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.4 14.3
-
 D-fructose pH 7, 37°C Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 7
-
-
 Entamoeba histolytica

Expression

EC Number Organism Comment Expression
2.7.1.4 Entamoeba histolytica fructokinase messenger RNA is modestly but significantly upregulated in amoebae switched to fructose medium as well as in fructose-adapted Entamoeba histolytica up