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Literature summary extracted from

  • Yang, J.; Xu, H.; Zhang, Y.; Bai, L.; Deng, Z.; Mahmud, T.
    Nucleotidylation of unsaturated carbasugar in validamycin biosynthesis (2011), Org. Biomol. Chem., 9, 438-449.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.91 expression in Escherichia coli Streptomyces hygroscopicus subsp. jinggangensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.91 Ca2+ able to rescue the activity of the EDTA-treated enzyme Streptomyces hygroscopicus subsp. jinggangensis
2.7.7.91 Mg2+ able to rescue the activity of the EDTA-treated enzyme Streptomyces hygroscopicus subsp. jinggangensis
2.7.7.91 Mn2+ able to rescue the activity of the EDTA-treated enzyme Streptomyces hygroscopicus subsp. jinggangensis
2.7.7.91 additional information divalent cation Mg2+, Mn2+, or Co2+ required, Fe2+, Cu2+, Ca2+, Zn2+, and Ni2+ are inactive Streptomyces hygroscopicus subsp. jinggangensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.91 45000
-
 x * 45000, SDS-PAGE Streptomyces hygroscopicus subsp. jinggangensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.91 GTP + valienol 1-phosphate Streptomyces hygroscopicus subsp. jinggangensis
-
 diphosphate + GDP-valienol
-
 ?
2.7.7.91 GTP + valienol 1-phosphate Streptomyces hygroscopicus subsp. jinggangensis 5008
-
 diphosphate + GDP-valienol
-
 ?
2.7.7.91 additional information Streptomyces hygroscopicus subsp. jinggangensis ValB efficiently catalyzes the conversion of valienol 1-phosphate to its nucleotidyl diphosphate derivatives, other unsaturated carbasugar phosphates are poor substrates. Purine-based nucleotidyltriphosphates ATP and GTP are more efficiently used as nucleotidyl donor than the pyrimidine-based CTP, dTTP, and UTP ?
-
 ?
2.7.7.91 additional information Streptomyces hygroscopicus subsp. jinggangensis 5008 ValB efficiently catalyzes the conversion of valienol 1-phosphate to its nucleotidyl diphosphate derivatives, other unsaturated carbasugar phosphates are poor substrates. Purine-based nucleotidyltriphosphates ATP and GTP are more efficiently used as nucleotidyl donor than the pyrimidine-based CTP, dTTP, and UTP ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.91 Streptomyces hygroscopicus subsp. jinggangensis Q3T6E3
-
-
2.7.7.91 Streptomyces hygroscopicus subsp. jinggangensis 5008 Q3T6E3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.91 ATP + D-glucose 1-phosphate
-
 Streptomyces hygroscopicus subsp. jinggangensis diphosphate + GDP-D-glucose
-
 ?
2.7.7.91 ATP + D-glucose 1-phosphate
-
 Streptomyces hygroscopicus subsp. jinggangensis 5008 diphosphate + GDP-D-glucose
-
 ?
2.7.7.91 CTP + valienol 1-phosphate
-
 Streptomyces hygroscopicus subsp. jinggangensis diphosphate + CDP-valienol
-
 ?
2.7.7.91 CTP + valienol 1-phosphate
-
 Streptomyces hygroscopicus subsp. jinggangensis 5008 diphosphate + CDP-valienol
-
 ?
2.7.7.91 GTP + valienol 1-phosphate
-
 Streptomyces hygroscopicus subsp. jinggangensis diphosphate + GDP-valienol
-
 ?
2.7.7.91 GTP + valienol 1-phosphate
-
 Streptomyces hygroscopicus subsp. jinggangensis 5008 diphosphate + GDP-valienol
-
 ?
2.7.7.91 additional information ValB efficiently catalyzes the conversion of valienol 1-phosphate to its nucleotidyl diphosphate derivatives, other unsaturated carbasugar phosphates are poor substrates. Purine-based nucleotidyltriphosphates ATP and GTP are more efficiently used as nucleotidyl donor than the pyrimidine-based CTP, dTTP, and UTP Streptomyces hygroscopicus subsp. jinggangensis ?
-
 ?
2.7.7.91 additional information ValB efficiently catalyzes the conversion of valienol 1-phosphate to its nucleotidyl diphosphate derivatives, other unsaturated carbasugar phosphates are poor substrates. Purine-based nucleotidyltriphosphates ATP and GTP are more efficiently used as nucleotidyl donor than the pyrimidine-based CTP, dTTP, and UTP Streptomyces hygroscopicus subsp. jinggangensis 5008 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.91 ? x * 45000, SDS-PAGE Streptomyces hygroscopicus subsp. jinggangensis

Synonyms

EC Number Synonyms Comment Organism
2.7.7.91 ValB
-
 Streptomyces hygroscopicus subsp. jinggangensis

General Information

EC Number General Information Comment Organism
2.7.7.91 physiological function inactivation of ValB abolishes production of validamycin A and validoxylamine A Streptomyces hygroscopicus subsp. jinggangensis