EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.B31 | 0.49 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus clavatus | |
2.5.1.34 | 0.47 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus fumigatus | |
2.5.1.34 | 0.9 | - |
L-m-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus fumigatus | |
2.5.1.80 | 0.17 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus fumigatus | |
2.5.1.122 | 0.58 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus niger |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.B31 | Ca2+ | activates, required | Aspergillus clavatus | |
2.5.1.34 | Ca2+ | activates | Aspergillus fumigatus | |
2.5.1.80 | Ca2+ | activates | Aspergillus fumigatus | |
2.5.1.122 | Ca2+ | activates | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.B31 | dimethylallyl diphosphate + L-tryptophan | Streptomyces coelicolor | - |
diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + L-tryptophan | Aspergillus clavatus | - |
diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + L-tryptophan | Aspergillus clavatus | C-5 prenylation | diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan | Aspergillus fumigatus | C-4 prenylation | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + L-tryptophan | Aspergillus fumigatus | C-7 prenylation | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.122 | dimethylallyl diphosphate + L-tyrosine | Aspergillus niger | O-4 prenylation | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.B31 | Aspergillus clavatus | - |
- |
- |
2.5.1.B31 | Streptomyces coelicolor | - |
- |
- |
2.5.1.34 | Aspergillus fumigatus | Q50EL0 | - |
- |
2.5.1.80 | Aspergillus fumigatus | Q4WYG3 | - |
- |
2.5.1.122 | Aspergillus niger | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.B31 | 2-pentenyl diphosphate + L-tryptophan | C-5, C-6 and C-7 prenylation | Streptomyces coelicolor | diphosphate + 5-(pent-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | 2-pentenyl diphosphate + L-tryptophan | C-5, C-6 and C-7 prenylation | Streptomyces coelicolor | diphosphate + 6-(pent-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | 2-pentenyl diphosphate + L-tryptophan | C-6 prenylation | Aspergillus clavatus | diphosphate + 6-(pent-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | 2-pentenyl diphosphate + L-tryptophan | C-5, C-6 and C-7 prenylation | Streptomyces coelicolor | diphosphate + 7-(pent-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | benzyl diphosphate + L-tryptophan | C-5 prenylation | Aspergillus clavatus | diphosphate + 5-benzyl-L-tryptophan | - |
? | |
2.5.1.B31 | benzyl diphosphate + L-tryptophan | C-6 prenylation | Streptomyces coelicolor | diphosphate + 6-benzyl-L-tryptophan | - |
? | |
2.5.1.B31 | benzyl diphosphate + L-tryptophan | C-6 prenylation | Aspergillus clavatus | diphosphate + 6-benzyl-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + 1-methyl-L-tryptophan | 46.9% of the activity with L-Trp | Aspergillus clavatus | diphosphate + 1-methyl-5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + 4-methyl-L-tryptophan | 56.6% of the activity with L-Trp | Aspergillus clavatus | diphosphate + 4-methyl-5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + 6-fluoro-L-tryptophan | 69.5% of the activity with L-Trp | Aspergillus clavatus | diphosphate + 6-fluoro-5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + 6-methyl-L-tryptophan | 53.2% of the activity with L-Trp | Aspergillus clavatus | diphosphate + 6-methyl-5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + 7-methyl-DL-tryptophan | 57.8% of the activity with L-Trp | Aspergillus clavatus | diphosphate + 7-methyl-5-(3-methylbut-2-enyl)-DL-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + beta-homo-L-tryptophan | 48.8% of the activity with L-Trp | Aspergillus clavatus | ? | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + DL-indole-3-lactic acid | 67.3% of the activity with L-Trp | Aspergillus clavatus | ? | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + indole-3-propionic acid | 70.7% of the activity with L-Trp | Aspergillus clavatus | ? | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + L-abrine | 90.9% of the activity with L-Trp | Aspergillus clavatus | ? | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + L-o-Tyr | C-3 prenylation | Aspergillus clavatus | diphosphate + 5-(3-methylbut-2-enyl)-L-o-tyrosine | 3-dimethylallyl-L-tyrosine | ? | |
2.5.1.B31 | dimethylallyl diphosphate + L-o-tyrosine | C-5 prenylation, proposed reaction mechanism, overview. Low activity | Aspergillus clavatus | diphosphate + 5-(3-methylbut-2-enyl)-L-o-tyrosine | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + L-tryptophan | - |
Streptomyces coelicolor | diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus clavatus | diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + L-tryptophan | best substrate | Streptomyces coelicolor | diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + L-tryptophan | best substrate | Aspergillus clavatus | diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + L-tryptophan | C-5 prenylation | Aspergillus clavatus | diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + N-acetyl-DL-tryptophan | 54.4% of the activity with L-Trp | Aspergillus clavatus | diphosphate + N-acetyl-5-(3-methylbut-2-enyl)-DL-tryptophan | - |
? | |
2.5.1.B31 | dimethylallyl diphosphate + trans-indole-3-acrylic acid | 38.0% of the activity with L-Trp | Aspergillus clavatus | ? | - |
? | |
2.5.1.B31 | methylallyl diphosphate + L-tryptophan | C-5 and C-6 prenylation | Aspergillus clavatus | diphosphate + 5-(but-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | methylallyl diphosphate + L-tryptophan | C-5, C-6 and C-7 prenylation | Streptomyces coelicolor | diphosphate + 5-(but-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | methylallyl diphosphate + L-tryptophan | C-5 and C-6 prenylation | Aspergillus clavatus | diphosphate + 6-(but-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | methylallyl diphosphate + L-tryptophan | C-5, C-6 and C-7 prenylation | Streptomyces coelicolor | diphosphate + 6-(but-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | methylallyl diphosphate + L-tryptophan | C-5, C-6 and C-7 prenylation | Streptomyces coelicolor | diphosphate + 7-(but-2-enyl)-L-tryptophan | - |
? | |
2.5.1.B31 | additional information | substrate specificity, overview | Streptomyces coelicolor | ? | - |
? | |
2.5.1.B31 | additional information | HR-ESI-MS and NMR product analysis. The enzyme catalyzes a regiospecific C-prenylation on the indole ring at C5 of L-Trp. No activity with L-m-tyrosine and L-tyrosine | Aspergillus clavatus | ? | - |
? | |
2.5.1.B31 | additional information | no or poor activity with 5-methyl-DL-Trp, 5-bromo-DL-Trp, L-m-Tyr. No activity with 6-[(2E)-pent-2-en-1-yl]-L-tryptophan. The enzyme catalyzes regiospecific prenylations of indolocarbazoles at the para-position of the indole N-atom | Aspergillus clavatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-m-tyrosine | C-4 and C-6 prenylation, proposed reaction mechanism, overview | Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-m-tyrosine + 6-(3-methylbut-2-enyl)-L-m-tyrosine | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-o-tyrosine | C-5 prenylation, proposed reaction mechanism, overview | Aspergillus fumigatus | diphosphate + 5-(3-methylbut-2-enyl)-L-o-tyrosine | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan | C-4 prenylation | Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-tyrosine | C-3 prenylation | Aspergillus fumigatus | diphosphate + 3-(3-methylbut-2-enyl)-L-tyrosine | - |
? | |
2.5.1.34 | additional information | HR-ESI-MS and NMR product analysis | Aspergillus fumigatus | ? | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + L-o-tyrosine | C-5 prenylation, proposed reaction mechanism, overview | Aspergillus fumigatus | diphosphate + 5-(3-methylbut-2-enyl)-L-o-tyrosine | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + L-tryptophan | C-7 prenylation | Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + L-tyrosine | O-4 prenylation | Aspergillus fumigatus | diphosphate + 5-[(2E)-pent-2-en-1-yl]-L-tryptophan | - |
? | |
2.5.1.80 | additional information | HR-ESI-MS and NMR product analysis. No activity with L-3-tyrosine | Aspergillus fumigatus | ? | - |
? | |
2.5.1.122 | dimethylallyl diphosphate + L-o-tyrosine | C-5 prenylation | Aspergillus niger | diphosphate + 5-(3-methylbut-2-enyl)-L-o-tyrosine | - |
? | |
2.5.1.122 | dimethylallyl diphosphate + L-tryptophan | C-7 prenylation | Aspergillus niger | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.122 | dimethylallyl diphosphate + L-tyrosine | O-4 prenylation | Aspergillus niger | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
2.5.1.122 | additional information | HR-ESI-MS and NMR product analysis. No activity with L-m-tyrosine | Aspergillus niger | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.B31 | 5-DMATS | - |
Streptomyces coelicolor |
2.5.1.B31 | 5-DMATS | - |
Aspergillus clavatus |
2.5.1.B31 | tryptophan C5-prenyltransferase | - |
Aspergillus clavatus |
2.5.1.34 | FgaPT2 | - |
Aspergillus fumigatus |
2.5.1.34 | tryptophan C4-prenyltransferase | - |
Aspergillus fumigatus |
2.5.1.80 | 7-DMATS | - |
Aspergillus fumigatus |
2.5.1.80 | tryptophan C7-prenyltransferase | - |
Aspergillus fumigatus |
2.5.1.122 | TyrPT | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.B31 | 37 | - |
assay at | Aspergillus clavatus |
2.5.1.34 | 37 | - |
assay at | Aspergillus fumigatus |
2.5.1.80 | 37 | - |
assay at | Aspergillus fumigatus |
2.5.1.122 | 37 | - |
assay at | Aspergillus niger |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.B31 | 0.013 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus clavatus | |
2.5.1.34 | 0.0065 | - |
L-m-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus fumigatus | |
2.5.1.34 | 0.29 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus fumigatus | |
2.5.1.80 | 0.045 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus fumigatus | |
2.5.1.122 | 0.014 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.B31 | 7.5 | - |
assay at | Aspergillus clavatus |
2.5.1.34 | 7.5 | - |
assay at | Aspergillus fumigatus |
2.5.1.80 | 7.5 | - |
assay at | Aspergillus fumigatus |
2.5.1.122 | 7.5 | - |
assay at | Aspergillus niger |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.B31 | evolution | the enzyme belongs to the DMATS superfamily | Aspergillus clavatus |
2.5.1.34 | evolution | the enzyme is part of the DMATS superfamily | Aspergillus fumigatus |
2.5.1.34 | metabolism | the enzyme is involved in the biosynthesis of fumigaclavine C | Aspergillus fumigatus |
2.5.1.80 | evolution | the enzyme is part of the DMATS superfamily | Aspergillus fumigatus |
2.5.1.80 | metabolism | the enzyme is involved in the biosynthesis of astechrome | Aspergillus fumigatus |
2.5.1.122 | evolution | the enzyme is part of the DMATS superfamily | Aspergillus niger |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.B31 | 0.0265 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus clavatus | |
2.5.1.34 | 0.0072 | - |
L-m-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus fumigatus | |
2.5.1.34 | 0.617 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus fumigatus | |
2.5.1.80 | 0.265 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus fumigatus | |
2.5.1.122 | 0.0241 | - |
L-o-tyrosine | pH 7.5, 37°C, recombinant enzyme | Aspergillus niger |