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Literature summary extracted from

  • Jiang, H.; Zhao, L.; Dong, X.; He, A.; Zheng, C.; Johansson, M.; Karlsson, A.; Zheng, X.
    Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria (2015), Oncol. Rep., 34, 1487-1493.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.145 medicine possible use of this enzyme as a suicide gene in vitro and in vivo Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.145 heterologous expression of Drosophila melanogaster enzyme Dm-dNK increases the sensitivity of cancer cells to several cytotoxic nucleoside analogues, recombinant expression of GFP-tagged mutant and wild-type enzymes in TK-1-deficient osteosarcoma cells. dThd kinase activities increased about 40fold in the cells expressing the nuclear dNK-GFP and about 35fold in the cells expressing the mitochondrial mito-dNK-GFP, compared with the untransduced parent cells Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.145 mitochondrion Dm-dNK localizes to the nucleus through a mitochondrial localization signal Drosophila melanogaster 5739
-
2.7.1.145 nucleus Dm-dNK localizes to the nucleus through a C-terminal nuclear localization signal Drosophila melanogaster 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.145 ATP + 2'-deoxyribonucleoside Drosophila melanogaster
-
 ADP + 2'-deoxyribonucleoside 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster Q9XZT6 gene dnk
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.145 carcinoma cell
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 ATP + 2'-deoxyribonucleoside
-
 Drosophila melanogaster ADP + 2'-deoxyribonucleoside 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine
-
 Drosophila melanogaster ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 additional information Dm-dNK phosphorylates a broad range of substrates including analogues of both purine and pyrimidine nucleosides, and exhibits a higher activity than other nucleoside kinases Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.145 Dm-dNK
-
 Drosophila melanogaster
2.7.1.145 multi-substrate deoxyribonucleoside kinase
-
 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.145 ATP
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.7.1.145 physiological function no differences between mutant cytosolic Dm-dNK, mitochondrial Dm-dNK, and wild-type nuclear Dm-dNK in regards to enzyme activity, cellular sensitivity to nucleoside analogues, or bystander cell killing. Tan IIA may influence the bystander effect of cancer cells expressing Dm-dNK by regulating the expression of Cx43 and Cx26. Tanshinone (Tan) IIA is a fat-soluble and pharmacologically active ingredient of Danshen, a traditional Chinese medicine used in the treatment of cardiovascular diseases. It is isolated from the rhizome of a Chinese herb Salvia miltiorrhiza. Tan IIA treatment of osteosarcoma cells results in the upregulation of Cx43 and Cx26 proteins Drosophila melanogaster