EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.88 | F1269A | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
2.7.7.88 | G1100A | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
2.7.7.88 | G1154A | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation and also reduces GTPase activity. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
2.7.7.88 | P1104A | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
2.7.7.88 | P1104V | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
2.7.7.88 | Q1270A | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
2.7.7.88 | T1157A | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
2.7.7.88 | T1157S | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
2.7.7.88 | W1188A | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation and also reduces GTPase activity. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
2.7.7.88 | Y1152A | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation and also reduces GTPase activity. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
2.7.7.88 | Y1152W | residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation and also reduces GTPase activity. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells | vesicular stomatitis virus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.88 | vesicular stomatitis virus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.88 | pppAACAG + GDP | oligo-RNa substrate. Residues G1100 in motif A, T1157 in motif B, W1188 in motif C, and F1269 and Q1270 in motif E are essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP | vesicular stomatitis virus | diphosphate + guanosine 5'-pppAACAG | - |
? |