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Literature summary extracted from

  • Yoo, H.; Widhalm, J.; Qian, Y.; Maeda, H.; Cooper, B.; Jannasch, A.; Gonda, I.; Lewinsohn, E.; Rhodes, D.; Dudareva, N.
    An alternative pathway contributes to phenylalanine biosynthesis in plants via a cytosolic tyrosine:phenylpyruvate aminotransferase (2013), Nat. Commun., 4, 2833.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 1.5
-
 phenylpyruvate cosubstrate L-tyrosine, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 1.8
-
 2-oxoglutarate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 1.9
-
 L-tyrosine cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 2.6
-
 phenylpyruvate cosubstrate L-glutamate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 4.3
-
 L-phenylalanine cosubstrate 4-hydroxyphenylpyruvate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 7.9
-
 4-hydroxyphenylpyruvate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 11.1
-
 L-glutamate cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 13.8
-
 L-phenylalanine cosubstrate 2-oxoglutarate, pH 10.0, 30°C Petunia x hybrida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.5 cytosol
-
 Petunia x hybrida 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Petunia x hybrida V5M241
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 2-oxoglutarate + L-phenylalanine
-
 Petunia x hybrida L-glutamate + phenylpyruvate
-
 ?
2.6.1.5 4-hydroxyphenylpyruvate + L-phenylalanine
-
 Petunia x hybrida L-glutamate + phenylpyruvate
-
 ?
2.6.1.5 L-glutamate + phenylpyruvate
-
 Petunia x hybrida 2-oxoglutarate + L-phenylalanine
-
 r
2.6.1.5 L-tyrosine + phenylpyruvate
-
 Petunia x hybrida 4-hydroxyphenylpyruvate + L-phenylalanine
-
 r
2.6.1.5 additional information activities with glutamate, methionine and leucine do not exceed 13% of the activity with tyrosine. Only phenylpyruvate and 2-oxoglutarate effectively serve as acceptors, and no activity is detected with prephenate Petunia x hybrida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.5 PPY-AT
-
 Petunia x hybrida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.5 0.17
-
 L-phenylalanine cosubstrate 4-hydroxyphenylpyruvate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.44
-
 L-phenylalanine cosubstrate 2-oxoglutarate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.44
-
 L-glutamate cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.7
-
 4-hydroxyphenylpyruvate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.92
-
 2-oxoglutarate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 1.19
-
 phenylpyruvate cosubstrate L-glutamate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 1.46
-
 phenylpyruvate cosubstrate L-tyrosine, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 1.48
-
 L-tyrosine cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.5 pyridoxal 5'-phosphate
-
 Petunia x hybrida

General Information

EC Number General Information Comment Organism
2.6.1.5 physiological function Petunia hybrida utilizes a microbial-like phenylpyruvate pathway to produce phenylalanine, and flux through this route is increased when the entry point to the arogenate pathway is limiting. The plant phenylpyruvate pathway utilizes cytosolic tyrosine aminotransferase that links the coordinated catabolism of tyrosine to serve as the amino donor. The enzyme is able to functionally complement the Escherichia coli phenylalanine auxotrophic mutant DL39 which lacks the three aminotransferases, AspC (aspartate aminotransferase), TyrB (L-Tyrosine aminotransferase) and IlvE (branched-chain aminotransferase) Petunia x hybrida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.5 0.04
-
 L-phenylalanine cosubstrate 2-oxoglutarate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.04
-
 L-phenylalanine cosubstrate 4-hydroxyphenylpyruvate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.04
-
 L-glutamate cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.09
-
 4-hydroxyphenylpyruvate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.46
-
 phenylpyruvate cosubstrate L-glutamate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.51
-
 2-oxoglutarate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.77
-
 L-tyrosine cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida
2.6.1.5 0.96
-
 phenylpyruvate cosubstrate L-tyrosine, pH 10.0, 30°C Petunia x hybrida