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Literature summary extracted from

  • Kim, E.J.; Oh, E.K.; Lee, J.K.
    Role of HemF and HemN in the heme biosynthesis of Vibrio vulnificus under S-adenosylmethionine-limiting conditions (2015), Mol. Microbiol., 96, 497-512.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.98.3 expressed in Escherichia coli BL21 cells Vibrio vulnificus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.98.3 Y74F the apparent binding affinity of the mutant enzyme for the cofactor S-adenosyl-L-methionine is higher than that of the wild type enzyme Vibrio vulnificus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.98.3 0.0052
-
 Coproporphyrinogen III wild type enzyme, at pH 8.0 and 30°C Vibrio vulnificus
1.3.98.3 0.0067
-
 Coproporphyrinogen III mutant enzyme Y74F, at pH 8.0 and 30°C Vibrio vulnificus
1.3.98.3 0.15
-
 S-adenosyl-L-methionine mutant enzyme Y74F, at pH 8.0 and 30°C Vibrio vulnificus
1.3.98.3 0.42
-
 S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 30°C Vibrio vulnificus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.98.3 55000
-
-
 Vibrio vulnificus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.98.3 coproporphyrinogen III + S-adenosyl-L-methionine Vibrio vulnificus
-
 protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine
-
 ?
1.3.98.3 coproporphyrinogen III + S-adenosyl-L-methionine Vibrio vulnificus ATCC 29307
-
 protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.98.3 Vibrio vulnificus
-
-
-
1.3.98.3 Vibrio vulnificus ATCC 29307
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.98.3 Strep-Tactin-Sepharose column chromatography Vibrio vulnificus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.98.3 coproporphyrinogen III + S-adenosyl-L-methionine
-
 Vibrio vulnificus protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine
-
 ?
1.3.98.3 coproporphyrinogen III + S-adenosyl-L-methionine
-
 Vibrio vulnificus ATCC 29307 protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.98.3 ? x * 55000, Strep-tagged enzyme, SDS-PAGE Vibrio vulnificus

Synonyms

EC Number Synonyms Comment Organism
1.3.98.3 coproporphyrinogen III oxidase
-
 Vibrio vulnificus
1.3.98.3 CPO
-
 Vibrio vulnificus
1.3.98.3 HemN
-
 Vibrio vulnificus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.98.3 360
-
 Coproporphyrinogen III mutant enzyme Y74F, at pH 8.0 and 30°C Vibrio vulnificus
1.3.98.3 900
-
 Coproporphyrinogen III wild type enzyme, at pH 8.0 and 30°C Vibrio vulnificus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.98.3 [4Fe-4S]-center
-
 Vibrio vulnificus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.98.3 54000
-
 Coproporphyrinogen III mutant enzyme Y74F, at pH 8.0 and 30°C Vibrio vulnificus
1.3.98.3 175000
-
 Coproporphyrinogen III wild type enzyme, at pH 8.0 and 30°C Vibrio vulnificus