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Literature summary extracted from

  • Balogun, E.O.; Inaoka, D.K.; Shiba, T.; Kido, Y.; Tsuge, C.; Nara, T.; Aoki, T.; Honma, T.; Tanaka, A.; Inoue, M.; Matsuoka, S.; Michels, P.A.; Kita, K.; Harada, S.
    Molecular basis for the reverse reaction of African human trypanosomes glycerol kinase (2014), Mol. Microbiol., 94, 1315-1329.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.30 in complex with substrates, sitting drop vapor diffusion method, using M HEPES pH 7.5, 11% (v/v) hexane-1,6-diol, 25% (w/v) PEG400 Trypanosoma brucei gambiense

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.30 D20A about 95% activity compared to the wild type enzyme Trypanosoma brucei gambiense
2.7.1.30 E478A about 25% activity compared to the wild type enzyme Trypanosoma brucei gambiense
2.7.1.30 M1A about 90% activity compared to the wild type enzyme Trypanosoma brucei gambiense
2.7.1.30 R22A about 60% activity compared to the wild type enzyme Trypanosoma brucei gambiense
2.7.1.30 R24A about 30% activity compared to the wild type enzyme Trypanosoma brucei gambiense
2.7.1.30 R477A inactive Trypanosoma brucei gambiense
2.7.1.30 T11V inactive Trypanosoma brucei gambiense
2.7.1.30 T12V less than 5% activity compared to the wild type enzyme Trypanosoma brucei gambiense
2.7.1.30 T273V about 80% activity compared to the wild type enzyme Trypanosoma brucei gambiense
2.7.1.30 T276V inactive Trypanosoma brucei gambiense
2.7.1.30 Y3F about 80% activity compared to the wild type enzyme Trypanosoma brucei gambiense

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.30 ADP + sn-glycerol 3-phosphate Trypanosoma brucei gambiense
-
 ATP + glycerol
-
 r
2.7.1.30 ATP + glycerol Trypanosoma brucei gambiense
-
 ADP + sn-glycerol 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Trypanosoma brucei gambiense D3KVM3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ADP + sn-glycerol 3-phosphate
-
 Trypanosoma brucei gambiense ATP + glycerol
-
 r
2.7.1.30 ATP + glycerol
-
 Trypanosoma brucei gambiense ADP + sn-glycerol 3-phosphate
-
 r