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Literature summary extracted from

  • Ling, C.S.; Yin, K.B.; Cun, S.T.; Ling, F.L.
    Expression profiling of choline and ethanolamine kinases in MCF7, HCT116 and HepG2 cells, and the transcriptional regulation by epigenetic modification (2015), Mol. Med. Rep., 11, 611-618.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.82 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.82 ATP + ethanolamine Homo sapiens
-
ADP + O-phosphoethanolamine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.82 Homo sapiens Q9HBU6 gene ek1; gene EKI1
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2.7.1.82 Homo sapiens Q9NVF9 gene eki2, splicing variants eki2alpha and eki2beta; gene eki2, splicing variants eki2alpha and eki2beta
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.82 HCT-116 cell
-
Homo sapiens
-
2.7.1.82 Hep-G2 cell
-
Homo sapiens
-
2.7.1.82 Hep-G2 cell expression levels of splicing variant ek2alpha is repressed in the HepG2 cells Homo sapiens
-
2.7.1.82 MCF-7 cell
-
Homo sapiens
-
2.7.1.82 additional information expression profile of the enzyme in cancer cell lines, overview Homo sapiens
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2.7.1.82 additional information expression profile of the enzyme in cancer cell lines, overview. Splice variant ek2beta exhibits a markedly low mRNA transcript level in all three of the cancer cell lines Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.82 ATP + ethanolamine
-
Homo sapiens ADP + O-phosphoethanolamine
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.82 ATP dependent on Homo sapiens

Expression

EC Number Organism Comment Expression
2.7.1.82 Homo sapiens substantial induction of splicing variant ek2alpha mRNA expression in response to trichostatin A treatment in Hep-G2 cells, the elevated ek2alpha mRNA expression may be due to the accumu­lation of acetylated histones H3 and H4, located around the promoter regions. Trichostatin A may also induce the recruit­ment of euchromatic markers and RNA polymerase II to the transcription factor complex that binds to the promoter, thus facilitating gene transcription up

General Information

EC Number General Information Comment Organism
2.7.1.82 evolution human ethanolamine kinase exists as three isoforms: EK1, EK2alpha and EK2beta, encoded by two separate genes; ek1, which produces the ek1 transcript variant 1, and ek2, which undergoes alternative splicing to produce ek2alpha (NCBI reference: NM_018208.2) and ek2beta (GenBank reference: AK001623.1) transcript variants. ek2alpha and ek2beta are respectively translated into EK2alpha and EK2beta Homo sapiens
2.7.1.82 metabolism choline kinase and ethanolamine kinase are enzymes that initiate the first step in the Kennedy pathway, resulting in the biosynthesis of phosphatidylcholine and phosphatidyletha­nolamine. Expression profiling of ethanolamine kinase in MCF-7, HCT-116 and Hep-G2 cells, and the transcriptional regulation by epigenetic modification Homo sapiens
2.7.1.82 physiological function splicing variant ek2beta exhibits a markedly low mRNA transcript level in all three of the cancer cell lines examined, thus suggesting that transcription of the ek2beta gene may be negligible and this isoform may not have a significant role in phosphatidyletanolamine synthesis in the three cancer cell lines Homo sapiens