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Literature summary extracted from

  • Lee, H.; Dutta, S.; Moon, I.S.
    Upregulation of dendritic arborization by N-acetyl-D-glucosamine kinase is not dependent on its kinase activity (2014), Mol. Cells, 37, 322-329.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.59 expressed in Escherichia coli BL21 (DE3)-pLysS cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.59 C143S the mutant retains 75% of wild type enzymatic activity Rattus norvegicus
2.7.1.59 D107A inactive Rattus norvegicus
2.7.1.59 N36A the mutation reduces enzyme activity to 51% of the wild type Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.59 0.13
-
 ATP wild type enzyme, at pH 7.5 and 37°C Rattus norvegicus
2.7.1.59 0.15
-
 ATP mutant enzyme C143S, at pH 7.5 and 37°C Rattus norvegicus
2.7.1.59 0.78
-
 N-acetyl-D-glucosamine wild type enzyme, at pH 7.5 and 37°C Rattus norvegicus
2.7.1.59 0.86
-
 N-acetyl-D-glucosamine mutant enzyme C143S, at pH 7.5 and 37°C Rattus norvegicus
2.7.1.59 1.17
-
 N-acetyl-D-glucosamine mutant enzyme N36A, at pH 7.5 and 37°C Rattus norvegicus
2.7.1.59 1.59
-
 ATP mutant enzyme N36A, at pH 7.5 and 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.59 40000
-
 x * 40000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine Rattus norvegicus
-
 ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.59 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.59 Ni-NTA column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.59 hippocampus
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine
-
 Rattus norvegicus ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.59 ? x * 40000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.59 GlcNAc kinase
-
 Rattus norvegicus
2.7.1.59 N-acetyl-D-glucosamine kinase
-
 Rattus norvegicus
2.7.1.59 NagK
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.7.1.59 physiological function the small domain of the enzyme, and not its kinase activity, plays a critical role in the upregulation of dendritogenesis Rattus norvegicus