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Literature summary extracted from

  • Laadan, B.; Wallace-Salinas, V.; Carlsson, A.J.; Almeida, J.R.; Radstroem, P.; Gorwa-Grauslund, M.F.
    Furaldehyde substrate specificity and kinetics of Saccharomyces cerevisiae alcohol dehydrogenase 1 variants (2014), Microb. Cell Fact., 13, 112.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.1 S110P/Y295C mutant is able to catalyze the NADH-dependent reduction of 5-hydroxymethylfurfural, an inhibitor of yeast fermentation, best activity among the mutants isolated Saccharomyces cerevisiae
1.1.1.1 Y295C mutant is able to catalyze the NADH-dependent reduction of 5-hydroxymethylfurfural, an inhibitor of yeast fermentation Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 5-hydroxymethylfurfural
-
Saccharomyces cerevisiae
1.1.1.1 acetaldehyde
-
Saccharomyces cerevisiae
1.1.1.1 furfural
-
Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.13
-
furfural mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
1.1.1.1 0.22
-
acetaldehyde wild-type, pH 6.7, 30°C Saccharomyces cerevisiae
1.1.1.1 0.83
-
acetaldehyde mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
1.1.1.1 9.45
-
5-hydroxymethylfurfural mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
1.1.1.1 18.8
-
furfural wild-type, pH 6.7, 30°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharomyces cerevisiae
-
-
-
1.1.1.1 Saccharomyces cerevisiae TMB3000
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 5-hydroxymethylfurfural + NADH + H+ no substrate for wild-type, reaction is catalyzed by mutants Y295C and S110P/Y295C Saccharomyces cerevisiae (furan-2,5-diyl)dimethanol + NAD+
-
?
1.1.1.1 5-hydroxymethylfurfural + NADH + H+ no substrate for wild-type, reaction is catalyzed by mutants Y295C and S110P/Y295C Saccharomyces cerevisiae TMB3000 (furan-2,5-diyl)dimethanol + NAD+
-
?
1.1.1.1 acetaldehyde + NADH + H+
-
Saccharomyces cerevisiae ethanol + NAD+
-
?
1.1.1.1 acetaldehyde + NADH + H+
-
Saccharomyces cerevisiae TMB3000 ethanol + NAD+
-
?
1.1.1.1 furfural + NADH + H+
-
Saccharomyces cerevisiae furfuryl alcohol + NAD+
-
?
1.1.1.1 furfural + NADH + H+
-
Saccharomyces cerevisiae TMB3000 furfuryl alcohol + NAD+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH1
-
Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.1 1.32
-
furfural mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
1.1.1.1 12.53
-
acetaldehyde wild-type, pH 6.7, 30°C Saccharomyces cerevisiae
1.1.1.1 20.8
-
5-hydroxymethylfurfural mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
1.1.1.1 46
-
acetaldehyde mutant S110P/Y295C, pH 6.7, 30°C Saccharomyces cerevisiae
1.1.1.1 1363
-
furfural wild-type, pH 6.7, 30°C Saccharomyces cerevisiae